Immunodominant Linear B-Cell Epitopes of SARS-CoV-2 Spike, Identified by Sera from K18-hACE2 Mice Infected with the WT or Variant Viruses
SARS-CoV-2 surface spike protein mediates the viral entry into the host cell and represents the primary immunological target of COVID-19 vaccines as well as post-exposure immunotherapy. Establishment of the highly immunogenic B-cell epitope profile of SARS-CoV-2 proteins in general, and that of the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/2/251 |
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| author | Yinon Levy Ron Alcalay Anat Zvi Efi Makdasi Eldar Peretz Tal Noy-Porat Theodor Chitlaru Michal Mandelboim Ohad Mazor Ronit Rosenfeld |
| author_facet | Yinon Levy Ron Alcalay Anat Zvi Efi Makdasi Eldar Peretz Tal Noy-Porat Theodor Chitlaru Michal Mandelboim Ohad Mazor Ronit Rosenfeld |
| author_sort | Yinon Levy |
| collection | DOAJ |
| description | SARS-CoV-2 surface spike protein mediates the viral entry into the host cell and represents the primary immunological target of COVID-19 vaccines as well as post-exposure immunotherapy. Establishment of the highly immunogenic B-cell epitope profile of SARS-CoV-2 proteins in general, and that of the spike protein in particular, may contribute to the development of sensitive diagnostic tools and identification of vaccine` candidate targets. In the current study, the anti-viral antibody response in transgenic K18-hACE-2 mice was examined by implementing an immunodominant epitope mapping approach of the SARS-CoV-2 spike. Serum samples for probing an epitope array covering the entire spike protein were collected from mice following infection with the original SARS-CoV-2 strain as well as the B.1.1.7 Alpha and B.1.351 Beta genetic variants of concern. The analysis resulted in distinction of six linear epitopes common to the humoral response against all virus variants inspected at a frequency of more than 20% of the serum samples. Finally, the universality of the response was probed by cross-protective in vitro experiments using plaque-reducing neutralization tests. The data presented here has important implications for prediction of the efficacy of immune countermeasures against emerging SARS-CoV-2 variants. |
| first_indexed | 2024-03-09T20:55:03Z |
| format | Article |
| id | doaj.art-f6d874276ff7471bb333a002d4ea3bbb |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T20:55:03Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-f6d874276ff7471bb333a002d4ea3bbb2023-11-23T22:26:02ZengMDPI AGVaccines2076-393X2022-02-0110225110.3390/vaccines10020251Immunodominant Linear B-Cell Epitopes of SARS-CoV-2 Spike, Identified by Sera from K18-hACE2 Mice Infected with the WT or Variant VirusesYinon Levy0Ron Alcalay1Anat Zvi2Efi Makdasi3Eldar Peretz4Tal Noy-Porat5Theodor Chitlaru6Michal Mandelboim7Ohad Mazor8Ronit Rosenfeld9Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 7410001, IsraelDepartment of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 7410001, IsraelDepartment of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 7410001, IsraelDepartment of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 7410001, IsraelDepartment of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 7410001, IsraelDepartment of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 7410001, IsraelDepartment of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 7410001, IsraelThe Central Virology Laboratory, Israel Ministry of Health, Tel Hashomer, Ramat Gan 5266202, IsraelDepartment of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 7410001, IsraelDepartment of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 7410001, IsraelSARS-CoV-2 surface spike protein mediates the viral entry into the host cell and represents the primary immunological target of COVID-19 vaccines as well as post-exposure immunotherapy. Establishment of the highly immunogenic B-cell epitope profile of SARS-CoV-2 proteins in general, and that of the spike protein in particular, may contribute to the development of sensitive diagnostic tools and identification of vaccine` candidate targets. In the current study, the anti-viral antibody response in transgenic K18-hACE-2 mice was examined by implementing an immunodominant epitope mapping approach of the SARS-CoV-2 spike. Serum samples for probing an epitope array covering the entire spike protein were collected from mice following infection with the original SARS-CoV-2 strain as well as the B.1.1.7 Alpha and B.1.351 Beta genetic variants of concern. The analysis resulted in distinction of six linear epitopes common to the humoral response against all virus variants inspected at a frequency of more than 20% of the serum samples. Finally, the universality of the response was probed by cross-protective in vitro experiments using plaque-reducing neutralization tests. The data presented here has important implications for prediction of the efficacy of immune countermeasures against emerging SARS-CoV-2 variants.https://www.mdpi.com/2076-393X/10/2/251COVID-19SARS-CoV-2epitope mappinglinear epitopesK18-hACE2 |
| spellingShingle | Yinon Levy Ron Alcalay Anat Zvi Efi Makdasi Eldar Peretz Tal Noy-Porat Theodor Chitlaru Michal Mandelboim Ohad Mazor Ronit Rosenfeld Immunodominant Linear B-Cell Epitopes of SARS-CoV-2 Spike, Identified by Sera from K18-hACE2 Mice Infected with the WT or Variant Viruses Vaccines COVID-19 SARS-CoV-2 epitope mapping linear epitopes K18-hACE2 |
| title | Immunodominant Linear B-Cell Epitopes of SARS-CoV-2 Spike, Identified by Sera from K18-hACE2 Mice Infected with the WT or Variant Viruses |
| title_full | Immunodominant Linear B-Cell Epitopes of SARS-CoV-2 Spike, Identified by Sera from K18-hACE2 Mice Infected with the WT or Variant Viruses |
| title_fullStr | Immunodominant Linear B-Cell Epitopes of SARS-CoV-2 Spike, Identified by Sera from K18-hACE2 Mice Infected with the WT or Variant Viruses |
| title_full_unstemmed | Immunodominant Linear B-Cell Epitopes of SARS-CoV-2 Spike, Identified by Sera from K18-hACE2 Mice Infected with the WT or Variant Viruses |
| title_short | Immunodominant Linear B-Cell Epitopes of SARS-CoV-2 Spike, Identified by Sera from K18-hACE2 Mice Infected with the WT or Variant Viruses |
| title_sort | immunodominant linear b cell epitopes of sars cov 2 spike identified by sera from k18 hace2 mice infected with the wt or variant viruses |
| topic | COVID-19 SARS-CoV-2 epitope mapping linear epitopes K18-hACE2 |
| url | https://www.mdpi.com/2076-393X/10/2/251 |
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