Comparison of nonsense-mediated mRNA decay efficiency in various murine tissues
<p>Abstract</p> <p>Background</p> <p>The Nonsense-Mediated mRNA Decay (NMD) pathway detects and degrades mRNAs containing premature termination codons, thereby preventing the accumulation of potentially detrimental truncated proteins. Intertissue variation in the effici...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2008-12-01
|
| Series: | BMC Genetics |
| Online Access: | http://www.biomedcentral.com/1471-2156/9/83 |
| _version_ | 1828827487596969984 |
|---|---|
| author | Mazoyer Sylvie Zhang Chang X Buisson Monique Magnin Delphine Anczuków Olga Fontanière Sandra Zetoune Almoutassem B |
| author_facet | Mazoyer Sylvie Zhang Chang X Buisson Monique Magnin Delphine Anczuków Olga Fontanière Sandra Zetoune Almoutassem B |
| author_sort | Mazoyer Sylvie |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The Nonsense-Mediated mRNA Decay (NMD) pathway detects and degrades mRNAs containing premature termination codons, thereby preventing the accumulation of potentially detrimental truncated proteins. Intertissue variation in the efficiency of this mechanism has been suggested, which could have important implications for the understanding of genotype-phenotype correlations in various genetic disorders. However, compelling evidence in favour of this hypothesis is lacking. Here, we have explored this question by measuring the ratio of mutant versus wild-type <it>Men1 </it>transcripts in thirteen tissues from mice carrying a heterozygous truncating mutation in the ubiquitously expressed <it>Men1 </it>gene.</p> <p>Results</p> <p>Significant differences were found between two groups of tissues. The first group, which includes testis, ovary, brain and heart, displays a strong decrease of the nonsense transcript (average ratio of 18% of mutant versus wild-type <it>Men1 </it>transcripts, identical to the value measured in murine embryonic fibroblasts). The second group, comprising lung, intestine and thymus, shows much less pronounced NMD (average ratio of 35%). Importantly, the extent of degradation by NMD does not correlate with the expression level of eleven genes encoding proteins involved in NMD or with the expression level of the <it>Men1 </it>gene.</p> <p>Conclusion</p> <p>Mouse models are an attractive option to evaluate the efficiency of NMD in multiple mammalian tissues and organs, given that it is much easier to obtain these from a mouse than from a single individual carrying a germline truncating mutation. In this study, we have uncovered in the thirteen different murine tissues that we examined up to a two-fold difference in NMD efficiency.</p> |
| first_indexed | 2024-12-12T15:01:37Z |
| format | Article |
| id | doaj.art-f713f7f24bf548938af53b73ecac541b |
| institution | Directory Open Access Journal |
| issn | 1471-2156 |
| language | English |
| last_indexed | 2024-12-12T15:01:37Z |
| publishDate | 2008-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genetics |
| spelling | doaj.art-f713f7f24bf548938af53b73ecac541b2022-12-22T00:20:48ZengBMCBMC Genetics1471-21562008-12-01918310.1186/1471-2156-9-83Comparison of nonsense-mediated mRNA decay efficiency in various murine tissuesMazoyer SylvieZhang Chang XBuisson MoniqueMagnin DelphineAnczuków OlgaFontanière SandraZetoune Almoutassem B<p>Abstract</p> <p>Background</p> <p>The Nonsense-Mediated mRNA Decay (NMD) pathway detects and degrades mRNAs containing premature termination codons, thereby preventing the accumulation of potentially detrimental truncated proteins. Intertissue variation in the efficiency of this mechanism has been suggested, which could have important implications for the understanding of genotype-phenotype correlations in various genetic disorders. However, compelling evidence in favour of this hypothesis is lacking. Here, we have explored this question by measuring the ratio of mutant versus wild-type <it>Men1 </it>transcripts in thirteen tissues from mice carrying a heterozygous truncating mutation in the ubiquitously expressed <it>Men1 </it>gene.</p> <p>Results</p> <p>Significant differences were found between two groups of tissues. The first group, which includes testis, ovary, brain and heart, displays a strong decrease of the nonsense transcript (average ratio of 18% of mutant versus wild-type <it>Men1 </it>transcripts, identical to the value measured in murine embryonic fibroblasts). The second group, comprising lung, intestine and thymus, shows much less pronounced NMD (average ratio of 35%). Importantly, the extent of degradation by NMD does not correlate with the expression level of eleven genes encoding proteins involved in NMD or with the expression level of the <it>Men1 </it>gene.</p> <p>Conclusion</p> <p>Mouse models are an attractive option to evaluate the efficiency of NMD in multiple mammalian tissues and organs, given that it is much easier to obtain these from a mouse than from a single individual carrying a germline truncating mutation. In this study, we have uncovered in the thirteen different murine tissues that we examined up to a two-fold difference in NMD efficiency.</p>http://www.biomedcentral.com/1471-2156/9/83 |
| spellingShingle | Mazoyer Sylvie Zhang Chang X Buisson Monique Magnin Delphine Anczuków Olga Fontanière Sandra Zetoune Almoutassem B Comparison of nonsense-mediated mRNA decay efficiency in various murine tissues BMC Genetics |
| title | Comparison of nonsense-mediated mRNA decay efficiency in various murine tissues |
| title_full | Comparison of nonsense-mediated mRNA decay efficiency in various murine tissues |
| title_fullStr | Comparison of nonsense-mediated mRNA decay efficiency in various murine tissues |
| title_full_unstemmed | Comparison of nonsense-mediated mRNA decay efficiency in various murine tissues |
| title_short | Comparison of nonsense-mediated mRNA decay efficiency in various murine tissues |
| title_sort | comparison of nonsense mediated mrna decay efficiency in various murine tissues |
| url | http://www.biomedcentral.com/1471-2156/9/83 |
| work_keys_str_mv | AT mazoyersylvie comparisonofnonsensemediatedmrnadecayefficiencyinvariousmurinetissues AT zhangchangx comparisonofnonsensemediatedmrnadecayefficiencyinvariousmurinetissues AT buissonmonique comparisonofnonsensemediatedmrnadecayefficiencyinvariousmurinetissues AT magnindelphine comparisonofnonsensemediatedmrnadecayefficiencyinvariousmurinetissues AT anczukowolga comparisonofnonsensemediatedmrnadecayefficiencyinvariousmurinetissues AT fontanieresandra comparisonofnonsensemediatedmrnadecayefficiencyinvariousmurinetissues AT zetounealmoutassemb comparisonofnonsensemediatedmrnadecayefficiencyinvariousmurinetissues |