Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing

Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing

The CRISPR/Cas system has emerged as a powerful and versatile genome engineering tool. Here the authors couple Cas9 to effector protein Exonuclease III via coiled-coil mediated interactions, termed CCExo, leading to increased deletion sizes and enhanced gene knock-out efficiencies in cell lines, pri...

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Bibliographic Details
Main Authors: Duško Lainšček, Vida Forstnerič, Veronika Mikolič, Špela Malenšek, Peter Pečan, Mojca Benčina, Matjaž Sever, Helena Podgornik, Roman Jerala
Format: Article
Language:English
Published: Nature Portfolio 2022-06-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-022-31386-1

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https://doi.org/10.1038/s41467-022-31386-1

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