Active H3K27me3 demethylation by KDM6B is required for normal development of bovine preimplantation embryos
The substantial epigenetic remodeling that occurs during early stages of mammalian embryonic development likely contributes to reprogramming the parental genomes from a differentiated to a totipotent state and activation of the embryonic genome. Trimethylation of lysine 27 of histone 3 (H3K27me3) is...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-12-01
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| Series: | Epigenetics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592294.2017.1403693 |
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| author | Nhi Chung Yanina S. Bogliotti Wei Ding Marcela Vilarino Kazuki Takahashi James L. Chitwood Richard M. Schultz Pablo J. Ross |
| author_facet | Nhi Chung Yanina S. Bogliotti Wei Ding Marcela Vilarino Kazuki Takahashi James L. Chitwood Richard M. Schultz Pablo J. Ross |
| author_sort | Nhi Chung |
| collection | DOAJ |
| description | The substantial epigenetic remodeling that occurs during early stages of mammalian embryonic development likely contributes to reprogramming the parental genomes from a differentiated to a totipotent state and activation of the embryonic genome. Trimethylation of lysine 27 of histone 3 (H3K27me3) is a repressive mark that undergoes global dynamic changes during preimplantation development of several species. To ascertain the role of H3K27me3 in bovine preimplantation development we perturbed the activity of KDM6B, which demethylates H3K27me3. Knockdown of maternal KDM6B mRNA inhibited the reduction in global levels of H3K27me3 from 2-cell to 8-cell embryo stages and compromised development to the blastocyst stage; embryos that developed to the blastocyst stage had fewer inner cell mass (ICM) and trophectoderm (TE) cells. In addition, the transcriptome of KDM6B knockdown embryos was altered at the 8-cell stage and characterized by downregulation of transcripts related to transcriptional regulation, chromatin remodeling, and protein catabolism. Inhibiting the catalytic activity of KDM6B with a specific small molecule inhibitor also prevented the global decrease in H3K27me3 and compromised development to the blastocyst stage. These results indicate that histone demethylation activity, mediated by KDM6B, is required for the global decrease in H3K27me3, correct activation of the embryonic genome, and development to the blastocyst stage in bovine embryos. |
| first_indexed | 2024-03-11T23:07:01Z |
| format | Article |
| id | doaj.art-f731d54c5203461e901b7f7a3c0462db |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:07:01Z |
| publishDate | 2017-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-f731d54c5203461e901b7f7a3c0462db2023-09-21T13:09:20ZengTaylor & Francis GroupEpigenetics1559-22941559-23082017-12-0112121048105610.1080/15592294.2017.14036931403693Active H3K27me3 demethylation by KDM6B is required for normal development of bovine preimplantation embryosNhi Chung0Yanina S. Bogliotti1Wei Ding2Marcela Vilarino3Kazuki Takahashi4James L. Chitwood5Richard M. Schultz6Pablo J. Ross7University of California DavisUniversity of California DavisUniversity of California DavisUniversity of California DavisUniversity of California DavisUniversity of California DavisUniversity of California DavisUniversity of California DavisThe substantial epigenetic remodeling that occurs during early stages of mammalian embryonic development likely contributes to reprogramming the parental genomes from a differentiated to a totipotent state and activation of the embryonic genome. Trimethylation of lysine 27 of histone 3 (H3K27me3) is a repressive mark that undergoes global dynamic changes during preimplantation development of several species. To ascertain the role of H3K27me3 in bovine preimplantation development we perturbed the activity of KDM6B, which demethylates H3K27me3. Knockdown of maternal KDM6B mRNA inhibited the reduction in global levels of H3K27me3 from 2-cell to 8-cell embryo stages and compromised development to the blastocyst stage; embryos that developed to the blastocyst stage had fewer inner cell mass (ICM) and trophectoderm (TE) cells. In addition, the transcriptome of KDM6B knockdown embryos was altered at the 8-cell stage and characterized by downregulation of transcripts related to transcriptional regulation, chromatin remodeling, and protein catabolism. Inhibiting the catalytic activity of KDM6B with a specific small molecule inhibitor also prevented the global decrease in H3K27me3 and compromised development to the blastocyst stage. These results indicate that histone demethylation activity, mediated by KDM6B, is required for the global decrease in H3K27me3, correct activation of the embryonic genome, and development to the blastocyst stage in bovine embryos.http://dx.doi.org/10.1080/15592294.2017.1403693cattleembryonic genome activationh3k27me3histone demethylationjmjd3preimplantation developmentreprogrammingtotipotency |
| spellingShingle | Nhi Chung Yanina S. Bogliotti Wei Ding Marcela Vilarino Kazuki Takahashi James L. Chitwood Richard M. Schultz Pablo J. Ross Active H3K27me3 demethylation by KDM6B is required for normal development of bovine preimplantation embryos Epigenetics cattle embryonic genome activation h3k27me3 histone demethylation jmjd3 preimplantation development reprogramming totipotency |
| title | Active H3K27me3 demethylation by KDM6B is required for normal development of bovine preimplantation embryos |
| title_full | Active H3K27me3 demethylation by KDM6B is required for normal development of bovine preimplantation embryos |
| title_fullStr | Active H3K27me3 demethylation by KDM6B is required for normal development of bovine preimplantation embryos |
| title_full_unstemmed | Active H3K27me3 demethylation by KDM6B is required for normal development of bovine preimplantation embryos |
| title_short | Active H3K27me3 demethylation by KDM6B is required for normal development of bovine preimplantation embryos |
| title_sort | active h3k27me3 demethylation by kdm6b is required for normal development of bovine preimplantation embryos |
| topic | cattle embryonic genome activation h3k27me3 histone demethylation jmjd3 preimplantation development reprogramming totipotency |
| url | http://dx.doi.org/10.1080/15592294.2017.1403693 |
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