Heparan Sulfate Proteoglycan: An Arbovirus Attachment Factor Integral to Mosquito Salivary Gland Ducts
Variants of the prototype Alphavirus, Sindbis (SINV), were used in per os infections of adult female mosquitoes to investigate arbovirus interaction with the salivary gland (SG). Infection of Aedine mosquitoes with AR339, a heparan sulfate proteoglycan (HSPG)-dependent variant, resulted in gross pat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-12-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/6/12/5182 |
| _version_ | 1818358986134519808 |
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| author | Kristen A. Ciano Jason J. Saredy Doria F. Bowers |
| author_facet | Kristen A. Ciano Jason J. Saredy Doria F. Bowers |
| author_sort | Kristen A. Ciano |
| collection | DOAJ |
| description | Variants of the prototype Alphavirus, Sindbis (SINV), were used in per os infections of adult female mosquitoes to investigate arbovirus interaction with the salivary gland (SG). Infection of Aedine mosquitoes with AR339, a heparan sulfate proteoglycan (HSPG)-dependent variant, resulted in gross pathology in the SG lateral lobes while infection with TR339, a HSPG-independent variant, resulted in minimal SG pathology. HSPG was detected in the internal ducts of the SG lateral lobes by immunolabeling but not in the median lobe, or beyond the triad structure and external ducts. Reports that human lactoferrin interacts with HSPG, suggested an interference with virus attachment to receptors on vertebrate cells. Pre-incubation of Aedes albopictus cultured C7-10 cells with bovine lactoferrin (bLF) followed by adsorption of SINV resulted in earlier and greater intensity of cytopathic response to TR339 compared with AR339. Following pre-treatment of C7-10 cells with bLF, plaques from tissue culture-adapted high-titer SINVTaV-GFP-TC were observed at 48 h post-infection (p.i.), while plaques from low-titer SINVTaV-GFP-TC were not observed until 120 h p.i. Confocal optics detected this reporter virus at 30 days p.i. in the SG proximal lateral lobe, a region of HSPG-immunolocalization. Altogether these data suggest an association between SINV and HSPG in the host mosquito. |
| first_indexed | 2024-12-13T20:37:43Z |
| format | Article |
| id | doaj.art-f734d6f16aee4f9494a56c873a1e7ed6 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-13T20:37:43Z |
| publishDate | 2014-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f734d6f16aee4f9494a56c873a1e7ed62022-12-21T23:32:14ZengMDPI AGViruses1999-49152014-12-016125182519710.3390/v6125182v6125182Heparan Sulfate Proteoglycan: An Arbovirus Attachment Factor Integral to Mosquito Salivary Gland DuctsKristen A. Ciano0Jason J. Saredy1Doria F. Bowers2Department of Biology, University of North Florida, Jacksonville, FL 32224, USADepartment of Biology, University of North Florida, Jacksonville, FL 32224, USADepartment of Biology, University of North Florida, Jacksonville, FL 32224, USAVariants of the prototype Alphavirus, Sindbis (SINV), were used in per os infections of adult female mosquitoes to investigate arbovirus interaction with the salivary gland (SG). Infection of Aedine mosquitoes with AR339, a heparan sulfate proteoglycan (HSPG)-dependent variant, resulted in gross pathology in the SG lateral lobes while infection with TR339, a HSPG-independent variant, resulted in minimal SG pathology. HSPG was detected in the internal ducts of the SG lateral lobes by immunolabeling but not in the median lobe, or beyond the triad structure and external ducts. Reports that human lactoferrin interacts with HSPG, suggested an interference with virus attachment to receptors on vertebrate cells. Pre-incubation of Aedes albopictus cultured C7-10 cells with bovine lactoferrin (bLF) followed by adsorption of SINV resulted in earlier and greater intensity of cytopathic response to TR339 compared with AR339. Following pre-treatment of C7-10 cells with bLF, plaques from tissue culture-adapted high-titer SINVTaV-GFP-TC were observed at 48 h post-infection (p.i.), while plaques from low-titer SINVTaV-GFP-TC were not observed until 120 h p.i. Confocal optics detected this reporter virus at 30 days p.i. in the SG proximal lateral lobe, a region of HSPG-immunolocalization. Altogether these data suggest an association between SINV and HSPG in the host mosquito.http://www.mdpi.com/1999-4915/6/12/5182Alphavirusheparan sulfate proteoglycanAedine mosquitoessalivary gland ductsSindbis virusGFP |
| spellingShingle | Kristen A. Ciano Jason J. Saredy Doria F. Bowers Heparan Sulfate Proteoglycan: An Arbovirus Attachment Factor Integral to Mosquito Salivary Gland Ducts Viruses Alphavirus heparan sulfate proteoglycan Aedine mosquitoes salivary gland ducts Sindbis virus GFP |
| title | Heparan Sulfate Proteoglycan: An Arbovirus Attachment Factor Integral to Mosquito Salivary Gland Ducts |
| title_full | Heparan Sulfate Proteoglycan: An Arbovirus Attachment Factor Integral to Mosquito Salivary Gland Ducts |
| title_fullStr | Heparan Sulfate Proteoglycan: An Arbovirus Attachment Factor Integral to Mosquito Salivary Gland Ducts |
| title_full_unstemmed | Heparan Sulfate Proteoglycan: An Arbovirus Attachment Factor Integral to Mosquito Salivary Gland Ducts |
| title_short | Heparan Sulfate Proteoglycan: An Arbovirus Attachment Factor Integral to Mosquito Salivary Gland Ducts |
| title_sort | heparan sulfate proteoglycan an arbovirus attachment factor integral to mosquito salivary gland ducts |
| topic | Alphavirus heparan sulfate proteoglycan Aedine mosquitoes salivary gland ducts Sindbis virus GFP |
| url | http://www.mdpi.com/1999-4915/6/12/5182 |
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