Principles and Practical Considerations for the Analysis of Disease-Associated Alternative Splicing Events Using the Gateway Cloning-Based Minigene Vectors pDESTsplice and pSpliceExpress

Principles and Practical Considerations for the Analysis of Disease-Associated Alternative Splicing Events Using the Gateway Cloning-Based Minigene Vectors pDESTsplice and pSpliceExpress

Splicing is an important RNA processing step. Genetic variations can alter the splicing process and thereby contribute to the development of various diseases. Alterations of the splicing pattern can be examined by gene expression analyses, by computational tools for predicting the effects of genetic...

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Bibliographic Details
Main Authors: Elena Putscher, Michael Hecker, Brit Fitzner, Peter Lorenz, Uwe Klaus Zettl
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:International Journal of Molecular Sciences
Subjects:
alternative splicing
RNA processing
splicing regulation
splicing reporter minigene assay
gateway cloning
pDESTsplice
Online Access:https://www.mdpi.com/1422-0067/22/10/5154

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https://www.mdpi.com/1422-0067/22/10/5154

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