Recombinant Globular Domain of TcpA Pilin from <i>Vibrio cholerae</i> El Tor: Recovery from Inclusion Bodies and Structural Characterization

Recombinant Globular Domain of TcpA Pilin from <i>Vibrio cholerae</i> El Tor: Recovery from Inclusion Bodies and Structural Characterization

The production of recombinant proteins in <i>Escherichia coli</i> cells is often hampered by aggregation of newly synthesized proteins and formation of inclusion bodies. Here we propose the use of transverse urea gradient electrophoresis (TUGE) in testing the capability of folding of a r...

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Bibliographic Details
Main Authors: Victor Marchenkov, Elena Dubovitskya, Nina Kotova, Igor Tuchkov, Nina Smirnova, Natalia Marchenko, Alexey Surin, Vladimir Filimonov, Gennady Semisotnov
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:Life
Subjects:
inclusion bodies
protein purification
protein refolding
protein thermodynamics
<i>Vibrio cholerae</i> pilin
Online Access:https://www.mdpi.com/2075-1729/12/11/1802

Internet

https://www.mdpi.com/2075-1729/12/11/1802

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