Context-dependent deposition and regulation of mRNAs in P-bodies
Cells respond to stress by remodeling their transcriptome through transcription and degradation. Xrn1p-dependent degradation in P-bodies is the most prevalent decay pathway, yet, P-bodies may facilitate not only decay, but also act as a storage compartment. However, which and how mRNAs are selected...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2018-01-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/29815 |
| _version_ | 1828107630549139456 |
|---|---|
| author | Congwei Wang Fabian Schmich Sumana Srivatsa Julie Weidner Niko Beerenwinkel Anne Spang |
| author_facet | Congwei Wang Fabian Schmich Sumana Srivatsa Julie Weidner Niko Beerenwinkel Anne Spang |
| author_sort | Congwei Wang |
| collection | DOAJ |
| description | Cells respond to stress by remodeling their transcriptome through transcription and degradation. Xrn1p-dependent degradation in P-bodies is the most prevalent decay pathway, yet, P-bodies may facilitate not only decay, but also act as a storage compartment. However, which and how mRNAs are selected into different degradation pathways and what determines the fate of any given mRNA in P-bodies remain largely unknown. We devised a new method to identify both common and stress-specific mRNA subsets associated with P-bodies. mRNAs targeted for degradation to P-bodies, decayed with different kinetics. Moreover, the localization of a specific set of mRNAs to P-bodies under glucose deprivation was obligatory to prevent decay. Depending on its client mRNA, the RNA-binding protein Puf5p either promoted or inhibited decay. Furthermore, the Puf5p-dependent storage of a subset of mRNAs in P-bodies under glucose starvation may be beneficial with respect to chronological lifespan. |
| first_indexed | 2024-04-11T10:34:09Z |
| format | Article |
| id | doaj.art-f774038236ce4bc7a6a3668106ace87d |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T10:34:09Z |
| publishDate | 2018-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-f774038236ce4bc7a6a3668106ace87d2022-12-22T04:29:21ZengeLife Sciences Publications LtdeLife2050-084X2018-01-01710.7554/eLife.29815Context-dependent deposition and regulation of mRNAs in P-bodiesCongwei Wang0https://orcid.org/0000-0003-2277-5002Fabian Schmich1Sumana Srivatsa2Julie Weidner3Niko Beerenwinkel4https://orcid.org/0000-0002-0573-6119Anne Spang5https://orcid.org/0000-0002-2387-6203Growth and Development, Biozentrum, University of Basel, Basel, SwitzerlandDepartment of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland; Swiss Institute of Bioinformatics, Basel, SwitzerlandDepartment of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland; Swiss Institute of Bioinformatics, Basel, SwitzerlandGrowth and Development, Biozentrum, University of Basel, Basel, SwitzerlandDepartment of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland; Swiss Institute of Bioinformatics, Basel, SwitzerlandGrowth and Development, Biozentrum, University of Basel, Basel, SwitzerlandCells respond to stress by remodeling their transcriptome through transcription and degradation. Xrn1p-dependent degradation in P-bodies is the most prevalent decay pathway, yet, P-bodies may facilitate not only decay, but also act as a storage compartment. However, which and how mRNAs are selected into different degradation pathways and what determines the fate of any given mRNA in P-bodies remain largely unknown. We devised a new method to identify both common and stress-specific mRNA subsets associated with P-bodies. mRNAs targeted for degradation to P-bodies, decayed with different kinetics. Moreover, the localization of a specific set of mRNAs to P-bodies under glucose deprivation was obligatory to prevent decay. Depending on its client mRNA, the RNA-binding protein Puf5p either promoted or inhibited decay. Furthermore, the Puf5p-dependent storage of a subset of mRNAs in P-bodies under glucose starvation may be beneficial with respect to chronological lifespan.https://elifesciences.org/articles/29815mRNA sortingprocessing bodiesstress responsemRNA decaylifespan |
| spellingShingle | Congwei Wang Fabian Schmich Sumana Srivatsa Julie Weidner Niko Beerenwinkel Anne Spang Context-dependent deposition and regulation of mRNAs in P-bodies eLife mRNA sorting processing bodies stress response mRNA decay lifespan |
| title | Context-dependent deposition and regulation of mRNAs in P-bodies |
| title_full | Context-dependent deposition and regulation of mRNAs in P-bodies |
| title_fullStr | Context-dependent deposition and regulation of mRNAs in P-bodies |
| title_full_unstemmed | Context-dependent deposition and regulation of mRNAs in P-bodies |
| title_short | Context-dependent deposition and regulation of mRNAs in P-bodies |
| title_sort | context dependent deposition and regulation of mrnas in p bodies |
| topic | mRNA sorting processing bodies stress response mRNA decay lifespan |
| url | https://elifesciences.org/articles/29815 |
| work_keys_str_mv | AT congweiwang contextdependentdepositionandregulationofmrnasinpbodies AT fabianschmich contextdependentdepositionandregulationofmrnasinpbodies AT sumanasrivatsa contextdependentdepositionandregulationofmrnasinpbodies AT julieweidner contextdependentdepositionandregulationofmrnasinpbodies AT nikobeerenwinkel contextdependentdepositionandregulationofmrnasinpbodies AT annespang contextdependentdepositionandregulationofmrnasinpbodies |