Understanding and Controlling Sialylation in a CHO Fc-Fusion Process.

Understanding and Controlling Sialylation in a CHO Fc-Fusion Process.

A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed. In order to more tightly control glycosylation profiles, we sought to identify the cause of variab...

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Bibliographic Details
Main Authors: Amanda M Lewis, William D Croughan, Nelly Aranibar, Alison G Lee, Bethanne Warrack, Nicholas R Abu-Absi, Rutva Patel, Barry Drew, Michael C Borys, Michael D Reily, Zheng Jian Li
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4911072?pdf=render

Internet

http://europepmc.org/articles/PMC4911072?pdf=render

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