Evaluation of Sugarcane (Saccharum officinarum L.) Somaclonals Tolerance to Salinity Via In Vitro and In Vivo
Tissue culture technique was used to obtain salt tolerant variants from embryogenic calluses of sugarcane (Saccharum sp. var. CP48-103) that cultured on a selective medium containing different levels of NaCl (0, 0.2, 0.4, 0.6, and 0.8% NaCl). A total of four plants regenerated from the tolerant call...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2011-06-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1978301916301644 |
| _version_ | 1811230399629623296 |
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| author | MAHMOUD SHOMEILI MAJID NABIPOUR MOSA MESKARBASHEE HAMID RAJABI MEMARI |
| author_facet | MAHMOUD SHOMEILI MAJID NABIPOUR MOSA MESKARBASHEE HAMID RAJABI MEMARI |
| author_sort | MAHMOUD SHOMEILI |
| collection | DOAJ |
| description | Tissue culture technique was used to obtain salt tolerant variants from embryogenic calluses of sugarcane (Saccharum sp. var. CP48-103) that cultured on a selective medium containing different levels of NaCl (0, 0.2, 0.4, 0.6, and 0.8% NaCl). A total of four plants regenerated from the tolerant calluses were selected but the best of them in vigor grown in in vitro and hydroponic systems under salinity stress to comparison with source variety. With increasing supply of NaCl in both systems, root growth was more adversely affected than was shoot growth. Chlorophyll contents showed a decreasing trend and dry matter yield of plants reduced but in a slow rate in tolerant somaclonal than source variety. The biochemical analysis showed that at high salt concentration, Cl− and Na+ content in shoot and root increased. With rising salt concentration from 0 to 0.8%, content of Cl− in shoot and root of tolerant variant changed lower than parent showed that this variant had genetic lowest ratio of shoot/root chloride and had minimum transport of Cl− to shoots. Also this variant had high content of Ca2+ in shoot and high K+/Na+ ratio at all salinity levels. Thus it probably has genetic potential to avoid harmful ions uptake. |
| first_indexed | 2024-04-12T10:28:47Z |
| format | Article |
| id | doaj.art-f79408dbbb6e4ad6ae0043bdcf2f30f6 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 |
| language | English |
| last_indexed | 2024-04-12T10:28:47Z |
| publishDate | 2011-06-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-f79408dbbb6e4ad6ae0043bdcf2f30f62022-12-22T03:36:55ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192011-06-01182919610.4308/hjb.18.2.91Evaluation of Sugarcane (Saccharum officinarum L.) Somaclonals Tolerance to Salinity Via In Vitro and In VivoMAHMOUD SHOMEILI0MAJID NABIPOUR1MOSA MESKARBASHEE2HAMID RAJABI MEMARI3Department of Agronomy, Sugarcane Research Center, Tosea Neishekar Co., Golestan Bolvar, Ahvaz, IranDepartment of Agronomy, College of Agriculture, Shahid Chamran University, Ahvaz, IranDepartment of Agronomy, College of Agriculture, Shahid Chamran University, Ahvaz, IranDepartment of Agronomy, College of Agriculture, Shahid Chamran University, Ahvaz, IranTissue culture technique was used to obtain salt tolerant variants from embryogenic calluses of sugarcane (Saccharum sp. var. CP48-103) that cultured on a selective medium containing different levels of NaCl (0, 0.2, 0.4, 0.6, and 0.8% NaCl). A total of four plants regenerated from the tolerant calluses were selected but the best of them in vigor grown in in vitro and hydroponic systems under salinity stress to comparison with source variety. With increasing supply of NaCl in both systems, root growth was more adversely affected than was shoot growth. Chlorophyll contents showed a decreasing trend and dry matter yield of plants reduced but in a slow rate in tolerant somaclonal than source variety. The biochemical analysis showed that at high salt concentration, Cl− and Na+ content in shoot and root increased. With rising salt concentration from 0 to 0.8%, content of Cl− in shoot and root of tolerant variant changed lower than parent showed that this variant had genetic lowest ratio of shoot/root chloride and had minimum transport of Cl− to shoots. Also this variant had high content of Ca2+ in shoot and high K+/Na+ ratio at all salinity levels. Thus it probably has genetic potential to avoid harmful ions uptake.http://www.sciencedirect.com/science/article/pii/S1978301916301644sugarcanesalinitysomaclonalin vivoin vitro |
| spellingShingle | MAHMOUD SHOMEILI MAJID NABIPOUR MOSA MESKARBASHEE HAMID RAJABI MEMARI Evaluation of Sugarcane (Saccharum officinarum L.) Somaclonals Tolerance to Salinity Via In Vitro and In Vivo Hayati Journal of Biosciences sugarcane salinity somaclonal in vivo in vitro |
| title | Evaluation of Sugarcane (Saccharum officinarum L.) Somaclonals Tolerance to Salinity Via In Vitro and In Vivo |
| title_full | Evaluation of Sugarcane (Saccharum officinarum L.) Somaclonals Tolerance to Salinity Via In Vitro and In Vivo |
| title_fullStr | Evaluation of Sugarcane (Saccharum officinarum L.) Somaclonals Tolerance to Salinity Via In Vitro and In Vivo |
| title_full_unstemmed | Evaluation of Sugarcane (Saccharum officinarum L.) Somaclonals Tolerance to Salinity Via In Vitro and In Vivo |
| title_short | Evaluation of Sugarcane (Saccharum officinarum L.) Somaclonals Tolerance to Salinity Via In Vitro and In Vivo |
| title_sort | evaluation of sugarcane saccharum officinarum l somaclonals tolerance to salinity via in vitro and in vivo |
| topic | sugarcane salinity somaclonal in vivo in vitro |
| url | http://www.sciencedirect.com/science/article/pii/S1978301916301644 |
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