An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model
Abstract The Zika virus (ZIKV) outbreak in the Americas and South Pacific poses a significant burden on human health because of ZIKV’s neurotropic effects in the course of fetal development. Vaccine candidates against ZIKV are coming online, but immunological tools to study anti-ZIKV responses in pr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2017-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-00193-w |
| _version_ | 1819040077284638720 |
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| author | Jasdave S. Chahal Tao Fang Andrew W. Woodham Omar F. Khan Jingjing Ling Daniel G. Anderson Hidde L. Ploegh |
| author_facet | Jasdave S. Chahal Tao Fang Andrew W. Woodham Omar F. Khan Jingjing Ling Daniel G. Anderson Hidde L. Ploegh |
| author_sort | Jasdave S. Chahal |
| collection | DOAJ |
| description | Abstract The Zika virus (ZIKV) outbreak in the Americas and South Pacific poses a significant burden on human health because of ZIKV’s neurotropic effects in the course of fetal development. Vaccine candidates against ZIKV are coming online, but immunological tools to study anti-ZIKV responses in preclinical models, particularly T cell responses, remain sparse. We deployed RNA nanoparticle technology to create a vaccine candidate that elicited ZIKV E protein-specific IgG responses in C57BL/6 mice as assayed by ELISA. Using this tool, we identified a unique H-2Db-restricted epitope to which there was a CD8+ T cell response in mice immunized with our modified dendrimer-based RNA nanoparticle vaccine. These results demonstrate that this approach can be used to evaluate new candidate antigens and identify immune correlates without the use of live virus. |
| first_indexed | 2024-12-21T09:03:22Z |
| format | Article |
| id | doaj.art-f7a59228dbcc45f49fb90892d8c11545 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T09:03:22Z |
| publishDate | 2017-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f7a59228dbcc45f49fb90892d8c115452022-12-21T19:09:26ZengNature PortfolioScientific Reports2045-23222017-03-01711910.1038/s41598-017-00193-wAn RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse modelJasdave S. Chahal0Tao Fang1Andrew W. Woodham2Omar F. Khan3Jingjing Ling4Daniel G. Anderson5Hidde L. Ploegh6Whitehead Institute for Biomedical ResearchWhitehead Institute for Biomedical ResearchWhitehead Institute for Biomedical ResearchThe Koch Institute for Integrative Cancer Research, Massachusetts Institute of TechnologyWhitehead Institute for Biomedical ResearchThe Koch Institute for Integrative Cancer Research, Massachusetts Institute of TechnologyWhitehead Institute for Biomedical ResearchAbstract The Zika virus (ZIKV) outbreak in the Americas and South Pacific poses a significant burden on human health because of ZIKV’s neurotropic effects in the course of fetal development. Vaccine candidates against ZIKV are coming online, but immunological tools to study anti-ZIKV responses in preclinical models, particularly T cell responses, remain sparse. We deployed RNA nanoparticle technology to create a vaccine candidate that elicited ZIKV E protein-specific IgG responses in C57BL/6 mice as assayed by ELISA. Using this tool, we identified a unique H-2Db-restricted epitope to which there was a CD8+ T cell response in mice immunized with our modified dendrimer-based RNA nanoparticle vaccine. These results demonstrate that this approach can be used to evaluate new candidate antigens and identify immune correlates without the use of live virus.https://doi.org/10.1038/s41598-017-00193-w |
| spellingShingle | Jasdave S. Chahal Tao Fang Andrew W. Woodham Omar F. Khan Jingjing Ling Daniel G. Anderson Hidde L. Ploegh An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model Scientific Reports |
| title | An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model |
| title_full | An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model |
| title_fullStr | An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model |
| title_full_unstemmed | An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model |
| title_short | An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model |
| title_sort | rna nanoparticle vaccine against zika virus elicits antibody and cd8 t cell responses in a mouse model |
| url | https://doi.org/10.1038/s41598-017-00193-w |
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