Responses of the Mushroom <i>Pleurotus ostreatus</i> under Different CO<sub>2</sub> Concentration by Comparative Proteomic Analyses
Background: <i>Pleurotus ostreatus</i> is a popular edible mushroom in East Asian markets. Research on the responses of <i>P. ostreatus</i> under different carbon dioxide concentrations is limited. Methods: Label-free LC-MS/MS quantitative proteomics analysis technique was ad...
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MDPI AG
2022-06-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/7/652 |
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| author | Rongmei Lin Lujun Zhang Xiuqing Yang Qiaozhen Li Chenxiao Zhang Lizhong Guo Hao Yu Hailong Yu |
| author_facet | Rongmei Lin Lujun Zhang Xiuqing Yang Qiaozhen Li Chenxiao Zhang Lizhong Guo Hao Yu Hailong Yu |
| author_sort | Rongmei Lin |
| collection | DOAJ |
| description | Background: <i>Pleurotus ostreatus</i> is a popular edible mushroom in East Asian markets. Research on the responses of <i>P. ostreatus</i> under different carbon dioxide concentrations is limited. Methods: Label-free LC-MS/MS quantitative proteomics analysis technique was adopted to obtain the protein expression profiles of <i>P. ostreatus</i> fruiting body pileus collected under different carbon dioxide concentrations. The Pearson correlation coefficient analysis and principal component analysis were performed to reveal the correlation among samples. The differentially expressed proteins (DEPs) were organized. Gene ontology analysis was performed to divide the DEPs into different metabolic processes and pathways. Results: The expansion of stipes was inhibited in the high CO<sub>2</sub> group compared with that in the low CO<sub>2</sub> group. There were 415 DEPs (131 up- and 284 down-regulated) in <i>P. ostreatus</i> PH11 treated with 1% CO<sub>2</sub> concentration compared with <i>P. ostreatus</i> under atmospheric conditions. Proteins related to hydrolase activity, including several amidohydrolases and cell wall synthesis proteins, were highly expressed under high CO<sub>2</sub> concentration. Most of the kinases and elongation factors were significantly down-regulated under high CO<sub>2</sub> concentration. The results suggest that the metabolic regulation and development processes were inhibited under high CO<sub>2</sub> concentrations. In addition, the sexual differentiation process protein Isp4 was inhibited under high CO<sub>2</sub> concentrations, indicating that the sexual reproductive process was also inhibited under high CO<sub>2</sub> concentrations, which is inconsistent with the small fruiting body pileus under high CO<sub>2</sub> concentrations. Conclusions: This research reports the proteome analysis of commercially relevant edible fungi <i>P. ostreatus</i> under different carbon dioxide concentrations. This study deepens our understanding of the mechanism for CO<sub>2</sub>-induced morphological change in the <i>P. ostreatus</i> fruiting body, which will facilitate the artificial cultivation of edible mushrooms. |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T03:18:28Z |
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| spelling | doaj.art-f7a62a1f89bb43bc8150b7ccab88bbe62023-12-03T15:15:26ZengMDPI AGJournal of Fungi2309-608X2022-06-018765210.3390/jof8070652Responses of the Mushroom <i>Pleurotus ostreatus</i> under Different CO<sub>2</sub> Concentration by Comparative Proteomic AnalysesRongmei Lin0Lujun Zhang1Xiuqing Yang2Qiaozhen Li3Chenxiao Zhang4Lizhong Guo5Hao Yu6Hailong Yu7National Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaShandong Provincial Key Laboratory of Applied Mycology, School of Life Sciences, Qingdao Agricultural University, 700 Changcheng Road, Chengyang District, Qingdao 266109, ChinaNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaShandong Provincial Key Laboratory of Applied Mycology, School of Life Sciences, Qingdao Agricultural University, 700 Changcheng Road, Chengyang District, Qingdao 266109, ChinaShandong Provincial Key Laboratory of Applied Mycology, School of Life Sciences, Qingdao Agricultural University, 700 Changcheng Road, Chengyang District, Qingdao 266109, ChinaNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaBackground: <i>Pleurotus ostreatus</i> is a popular edible mushroom in East Asian markets. Research on the responses of <i>P. ostreatus</i> under different carbon dioxide concentrations is limited. Methods: Label-free LC-MS/MS quantitative proteomics analysis technique was adopted to obtain the protein expression profiles of <i>P. ostreatus</i> fruiting body pileus collected under different carbon dioxide concentrations. The Pearson correlation coefficient analysis and principal component analysis were performed to reveal the correlation among samples. The differentially expressed proteins (DEPs) were organized. Gene ontology analysis was performed to divide the DEPs into different metabolic processes and pathways. Results: The expansion of stipes was inhibited in the high CO<sub>2</sub> group compared with that in the low CO<sub>2</sub> group. There were 415 DEPs (131 up- and 284 down-regulated) in <i>P. ostreatus</i> PH11 treated with 1% CO<sub>2</sub> concentration compared with <i>P. ostreatus</i> under atmospheric conditions. Proteins related to hydrolase activity, including several amidohydrolases and cell wall synthesis proteins, were highly expressed under high CO<sub>2</sub> concentration. Most of the kinases and elongation factors were significantly down-regulated under high CO<sub>2</sub> concentration. The results suggest that the metabolic regulation and development processes were inhibited under high CO<sub>2</sub> concentrations. In addition, the sexual differentiation process protein Isp4 was inhibited under high CO<sub>2</sub> concentrations, indicating that the sexual reproductive process was also inhibited under high CO<sub>2</sub> concentrations, which is inconsistent with the small fruiting body pileus under high CO<sub>2</sub> concentrations. Conclusions: This research reports the proteome analysis of commercially relevant edible fungi <i>P. ostreatus</i> under different carbon dioxide concentrations. This study deepens our understanding of the mechanism for CO<sub>2</sub>-induced morphological change in the <i>P. ostreatus</i> fruiting body, which will facilitate the artificial cultivation of edible mushrooms.https://www.mdpi.com/2309-608X/8/7/652<i>P. ostreatus</i>proteomicscarbon dioxidefruiting bodyedible mushroom |
| spellingShingle | Rongmei Lin Lujun Zhang Xiuqing Yang Qiaozhen Li Chenxiao Zhang Lizhong Guo Hao Yu Hailong Yu Responses of the Mushroom <i>Pleurotus ostreatus</i> under Different CO<sub>2</sub> Concentration by Comparative Proteomic Analyses Journal of Fungi <i>P. ostreatus</i> proteomics carbon dioxide fruiting body edible mushroom |
| title | Responses of the Mushroom <i>Pleurotus ostreatus</i> under Different CO<sub>2</sub> Concentration by Comparative Proteomic Analyses |
| title_full | Responses of the Mushroom <i>Pleurotus ostreatus</i> under Different CO<sub>2</sub> Concentration by Comparative Proteomic Analyses |
| title_fullStr | Responses of the Mushroom <i>Pleurotus ostreatus</i> under Different CO<sub>2</sub> Concentration by Comparative Proteomic Analyses |
| title_full_unstemmed | Responses of the Mushroom <i>Pleurotus ostreatus</i> under Different CO<sub>2</sub> Concentration by Comparative Proteomic Analyses |
| title_short | Responses of the Mushroom <i>Pleurotus ostreatus</i> under Different CO<sub>2</sub> Concentration by Comparative Proteomic Analyses |
| title_sort | responses of the mushroom i pleurotus ostreatus i under different co sub 2 sub concentration by comparative proteomic analyses |
| topic | <i>P. ostreatus</i> proteomics carbon dioxide fruiting body edible mushroom |
| url | https://www.mdpi.com/2309-608X/8/7/652 |
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