Computational stabilization of T cell receptors allows pairing with antibodies to form bispecifics
Recombinant T-cells receptors can redirect naïve T cells but often have low or uneven expression. Here, the authors model mutations in TCR constant domains to increase T cell receptor expression, assembly, and stability and generate bispecific molecules.
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-05-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-16231-7 |
| _version_ | 1818992731227160576 |
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| author | Karen Froning Jack Maguire Arlene Sereno Flora Huang Shawn Chang Kenneth Weichert Anton J. Frommelt Jessica Dong Xiufeng Wu Heather Austin Elaine M. Conner Jonathan R. Fitchett Aik Roy Heng Deepa Balasubramaniam Mark T. Hilgers Brian Kuhlman Stephen J. Demarest |
| author_facet | Karen Froning Jack Maguire Arlene Sereno Flora Huang Shawn Chang Kenneth Weichert Anton J. Frommelt Jessica Dong Xiufeng Wu Heather Austin Elaine M. Conner Jonathan R. Fitchett Aik Roy Heng Deepa Balasubramaniam Mark T. Hilgers Brian Kuhlman Stephen J. Demarest |
| author_sort | Karen Froning |
| collection | DOAJ |
| description | Recombinant T-cells receptors can redirect naïve T cells but often have low or uneven expression. Here, the authors model mutations in TCR constant domains to increase T cell receptor expression, assembly, and stability and generate bispecific molecules. |
| first_indexed | 2024-12-20T20:30:49Z |
| format | Article |
| id | doaj.art-f7c7bcb62ff841d9828b8f1c2709f751 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-20T20:30:49Z |
| publishDate | 2020-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-f7c7bcb62ff841d9828b8f1c2709f7512022-12-21T19:27:22ZengNature PortfolioNature Communications2041-17232020-05-0111111410.1038/s41467-020-16231-7Computational stabilization of T cell receptors allows pairing with antibodies to form bispecificsKaren Froning0Jack Maguire1Arlene Sereno2Flora Huang3Shawn Chang4Kenneth Weichert5Anton J. Frommelt6Jessica Dong7Xiufeng Wu8Heather Austin9Elaine M. Conner10Jonathan R. Fitchett11Aik Roy Heng12Deepa Balasubramaniam13Mark T. Hilgers14Brian Kuhlman15Stephen J. Demarest16Eli Lilly Biotechnology Center, 10300 Campus Point DriveProgram in Bioinformatics and Computational Biology, University of North Carolina at Chapel HillEli Lilly Biotechnology Center, 10300 Campus Point DriveEli Lilly Biotechnology Center, 10300 Campus Point DriveEli Lilly Biotechnology Center, 10300 Campus Point DriveEli Lilly Biotechnology Center, 10300 Campus Point DriveEli Lilly Biotechnology Center, 10300 Campus Point DriveEli Lilly Biotechnology Center, 10300 Campus Point DriveEli Lilly Biotechnology Center, 10300 Campus Point DriveEli Lilly Biotechnology Center, 10300 Campus Point DriveEli Lilly Biotechnology Center, 10300 Campus Point DriveEli Lilly Biotechnology Center, 10300 Campus Point DriveEli Lilly Biotechnology Center, 10300 Campus Point DriveEli Lilly Biotechnology Center, 10300 Campus Point DriveEli Lilly Biotechnology Center, 10300 Campus Point DriveDepartment of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel HillEli Lilly Biotechnology Center, 10300 Campus Point DriveRecombinant T-cells receptors can redirect naïve T cells but often have low or uneven expression. Here, the authors model mutations in TCR constant domains to increase T cell receptor expression, assembly, and stability and generate bispecific molecules.https://doi.org/10.1038/s41467-020-16231-7 |
| spellingShingle | Karen Froning Jack Maguire Arlene Sereno Flora Huang Shawn Chang Kenneth Weichert Anton J. Frommelt Jessica Dong Xiufeng Wu Heather Austin Elaine M. Conner Jonathan R. Fitchett Aik Roy Heng Deepa Balasubramaniam Mark T. Hilgers Brian Kuhlman Stephen J. Demarest Computational stabilization of T cell receptors allows pairing with antibodies to form bispecifics Nature Communications |
| title | Computational stabilization of T cell receptors allows pairing with antibodies to form bispecifics |
| title_full | Computational stabilization of T cell receptors allows pairing with antibodies to form bispecifics |
| title_fullStr | Computational stabilization of T cell receptors allows pairing with antibodies to form bispecifics |
| title_full_unstemmed | Computational stabilization of T cell receptors allows pairing with antibodies to form bispecifics |
| title_short | Computational stabilization of T cell receptors allows pairing with antibodies to form bispecifics |
| title_sort | computational stabilization of t cell receptors allows pairing with antibodies to form bispecifics |
| url | https://doi.org/10.1038/s41467-020-16231-7 |
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