Prokaryotic expression and solubilisation of Arabidopsis ROOT UVB SENSITIVE 1 from inclusion bodies in Escherichia coli

Prokaryotic expression and solubilisation of Arabidopsis ROOT UVB SENSITIVE 1 from inclusion bodies in Escherichia coli

The RUS (ROOT UVB SENSITIVE 1) proteins characterized by their unique DUF647 domain are widely distributed in eukaryotes. Their functional roles are largely unknown except for the possible involvement of Arabidopsis RUS1 and RUS2 in early seedling development. To investigate the biochemical roles...

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Bibliographic Details
Main Authors: Xue-Wen Hou, Hong-Yun Tong, Zheng-Hui He
Format: Article
Language:English
Published: University of Belgrade 2022-04-01
Series:Botanica Serbica
Subjects:
seamless cloning
inclusion body
renaturation
protein purification
western blot
Online Access:https://botanicaserbica.bio.bg.ac.rs/arhiva/pdf/2022_46_1_827_full.pdf

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https://botanicaserbica.bio.bg.ac.rs/arhiva/pdf/2022_46_1_827_full.pdf

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