Reconstitution of the antagonistic effect between C1orf112/FIRRM-FIGNL1 and BRCA2 on RAD51 filament stabilization

Reconstitution of the antagonistic effect between C1orf112/FIRRM-FIGNL1 and BRCA2 on RAD51 filament stabilization

Summary: C1orf112/FIRRM is a recently identified DNA damage repair factor that regulates RAD51 in homologous recombination through interacting with the anti-recombinase FIGNL1. Here, we describe steps for purifying C1orf112/FIRRM, FIGNL1, miBRCA2, and RAD51 proteins from Escherichia coli or Saccharo...

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Bibliographic Details
Main Authors: Zenan Zhou, Han Yang, Xinxin Liang, Tao Zhou, Qixiang Liu, Jiadong Wang, Weibin Wang
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Molecular Biology
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672300758X

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http://www.sciencedirect.com/science/article/pii/S266616672300758X

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