Plant production of high affinity nanobodies that block SARS-CoV-2 spike protein binding with its receptor, human angiotensin converting enzyme
Nanobodies® (VHH antibodies), are small peptides that represent the antigen binding domain, VHH of unique single domain antibodies (heavy chain only antibodies, HcAb) derived from camelids. Here, we demonstrate production of VHH nanobodies against the SARS-CoV-2 spike proteins in the solanaceous pla...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1045337/full |
| _version_ | 1797978395865251840 |
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| author | Marco Pitino Laura A. Fleites Lauren Shrum Michelle Heck Robert G. Shatters |
| author_facet | Marco Pitino Laura A. Fleites Lauren Shrum Michelle Heck Robert G. Shatters |
| author_sort | Marco Pitino |
| collection | DOAJ |
| description | Nanobodies® (VHH antibodies), are small peptides that represent the antigen binding domain, VHH of unique single domain antibodies (heavy chain only antibodies, HcAb) derived from camelids. Here, we demonstrate production of VHH nanobodies against the SARS-CoV-2 spike proteins in the solanaceous plant Nicotiana benthamiana through transient expression and their subsequent detection verified through western blot. We demonstrate that these nanobodies competitively inhibit binding between the SARS-CoV-2 spike protein receptor binding domain and its human receptor protein, angiotensin converting enzyme 2. There has been significant interest and a number of publications on the use of plants as biofactories and even some reports of producing nanobodies in plants. Our data demonstrate that functional nanobodies blocking a process necessary to initiate SARS-CoV-2 infection into mammalian cells can be produced in plants. This opens the alternative of using plants in a scheme to rapidly respond to therapeutic needs for emerging pathogens in human medicine and agriculture. |
| first_indexed | 2024-04-11T05:22:17Z |
| format | Article |
| id | doaj.art-f7f96d4137f146d994ceadd193dab40c |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-11T05:22:17Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-f7f96d4137f146d994ceadd193dab40c2022-12-23T18:02:11ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-12-011010.3389/fbioe.2022.10453371045337Plant production of high affinity nanobodies that block SARS-CoV-2 spike protein binding with its receptor, human angiotensin converting enzymeMarco Pitino0Laura A. Fleites1Lauren Shrum2Michelle Heck3Robert G. Shatters4AgroSource, Inc, Jupiter, FL, United StatesAgroSource, Inc, Jupiter, FL, United StatesAgroSource, Inc, Jupiter, FL, United StatesEmerging Pests and Pathogens Research Unit, USDA Agricultural Research Service, Ithaca, NY, United StatesU.S. Horticultural Research Laboratory, Subtropical Insects and Horticulture Research Unit, USDA Agricultural Research Service, Fort Pierce, FL, United StatesNanobodies® (VHH antibodies), are small peptides that represent the antigen binding domain, VHH of unique single domain antibodies (heavy chain only antibodies, HcAb) derived from camelids. Here, we demonstrate production of VHH nanobodies against the SARS-CoV-2 spike proteins in the solanaceous plant Nicotiana benthamiana through transient expression and their subsequent detection verified through western blot. We demonstrate that these nanobodies competitively inhibit binding between the SARS-CoV-2 spike protein receptor binding domain and its human receptor protein, angiotensin converting enzyme 2. There has been significant interest and a number of publications on the use of plants as biofactories and even some reports of producing nanobodies in plants. Our data demonstrate that functional nanobodies blocking a process necessary to initiate SARS-CoV-2 infection into mammalian cells can be produced in plants. This opens the alternative of using plants in a scheme to rapidly respond to therapeutic needs for emerging pathogens in human medicine and agriculture.https://www.frontiersin.org/articles/10.3389/fbioe.2022.1045337/fullSARS-CoV-2nanobodyplantVHHcompetitive ELISAACE2 |
| spellingShingle | Marco Pitino Laura A. Fleites Lauren Shrum Michelle Heck Robert G. Shatters Plant production of high affinity nanobodies that block SARS-CoV-2 spike protein binding with its receptor, human angiotensin converting enzyme Frontiers in Bioengineering and Biotechnology SARS-CoV-2 nanobody plant VHH competitive ELISA ACE2 |
| title | Plant production of high affinity nanobodies that block SARS-CoV-2 spike protein binding with its receptor, human angiotensin converting enzyme |
| title_full | Plant production of high affinity nanobodies that block SARS-CoV-2 spike protein binding with its receptor, human angiotensin converting enzyme |
| title_fullStr | Plant production of high affinity nanobodies that block SARS-CoV-2 spike protein binding with its receptor, human angiotensin converting enzyme |
| title_full_unstemmed | Plant production of high affinity nanobodies that block SARS-CoV-2 spike protein binding with its receptor, human angiotensin converting enzyme |
| title_short | Plant production of high affinity nanobodies that block SARS-CoV-2 spike protein binding with its receptor, human angiotensin converting enzyme |
| title_sort | plant production of high affinity nanobodies that block sars cov 2 spike protein binding with its receptor human angiotensin converting enzyme |
| topic | SARS-CoV-2 nanobody plant VHH competitive ELISA ACE2 |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1045337/full |
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