Monosubstituted tricationic Zn(II) phthalocyanine enhances antimicrobial photodynamic inactivation (aPDI) of methicillin-resistant Staphylococcus aureus (MRSA) and cytotoxicity evaluation for topical applications: in vitro and in vivo study
ABSTRACTAntimicrobial photodynamic therapy (aPDT) is an innovative approach to combat multi-drug resistant bacteria. It is known that cationic Zn(II) phthalocyanines (ZnPc) are effective in mediating aPDT against methicillin-resistant Staphylococcus aureus (MRSA). Here we used ZnPc-based photosensit...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1790305 |
| _version_ | 1797265841266360320 |
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| author | Priyanga Dharmaratne Baiyan Wang Roy C. H. Wong Ben C. L. Chan Kit-Man Lau Mei-Rong Ke Clara B. S. Lau Dennis K. P. Ng Kwok-Pui Fung Margaret Ip |
| author_facet | Priyanga Dharmaratne Baiyan Wang Roy C. H. Wong Ben C. L. Chan Kit-Man Lau Mei-Rong Ke Clara B. S. Lau Dennis K. P. Ng Kwok-Pui Fung Margaret Ip |
| author_sort | Priyanga Dharmaratne |
| collection | DOAJ |
| description | ABSTRACTAntimicrobial photodynamic therapy (aPDT) is an innovative approach to combat multi-drug resistant bacteria. It is known that cationic Zn(II) phthalocyanines (ZnPc) are effective in mediating aPDT against methicillin-resistant Staphylococcus aureus (MRSA). Here we used ZnPc-based photosensitizer named ZnPcE previously reported by our research group to evaluate its aPDT efficacy against broad spectrum of clinically relevant MRSAs. Remarkably, in vitro anti-MRSA activity was achieved using near-infrared (NIR, >610 nm) light with minimal bactericidal concentrations ranging <0.019–0.156 µM against the panel of MRSAs. ZnPcE was not only significantly (p < .05) more potent than methylene blue, which is a clinically approved photosensitizer but also demonstrated low cytotoxicity against human fibroblasts cell line (Hs-27) and human immortalized keratinocytes cell line (HaCaT). The toxicity was further evaluated on human 3-D skin constructs and found ZnPcE did not manifest in vivo skin irritation at ≤7.8 µM concentration. In the murine MRSA wound model, ZnPcE with PDT group demonstrated > 4 log10 CFU reduction and the value is significantly higher (p < .05) than all test groups except positive control. To conclude, results of present study provide a scientific basis for future clinical evaluation of ZnPcE-PDT on MRSA wound infection. |
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| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-04-25T00:51:12Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
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| series | Emerging Microbes and Infections |
| spelling | doaj.art-f80083ce84494b2aabbd8f1b02d7c42f2024-03-11T16:04:24ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-01911628163710.1080/22221751.2020.1790305Monosubstituted tricationic Zn(II) phthalocyanine enhances antimicrobial photodynamic inactivation (aPDI) of methicillin-resistant Staphylococcus aureus (MRSA) and cytotoxicity evaluation for topical applications: in vitro and in vivo studyPriyanga Dharmaratne0Baiyan Wang1Roy C. H. Wong2Ben C. L. Chan3Kit-Man Lau4Mei-Rong Ke5Clara B. S. Lau6Dennis K. P. Ng7Kwok-Pui Fung8Margaret Ip9School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong (SAR), People’s Republic of ChinaSchool of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong (SAR), People’s Republic of ChinaDepartment of Chemistry, Faculty of Science, The Chinese University of Hong Kong, Hong Kong (SAR), People’s Republic of ChinaInstitute of Chinese Medicine and State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants, The Chinese University of Hong Kong, Hong Kong (SAR), People’s Republic of ChinaInstitute of Chinese Medicine and State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants, The Chinese University of Hong Kong, Hong Kong (SAR), People’s Republic of ChinaDepartment of Chemistry, Faculty of Science, The Chinese University of Hong Kong, Hong Kong (SAR), People’s Republic of ChinaInstitute of Chinese Medicine and State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants, The Chinese University of Hong Kong, Hong Kong (SAR), People’s Republic of ChinaDepartment of Chemistry, Faculty of Science, The Chinese University of Hong Kong, Hong Kong (SAR), People’s Republic of ChinaSchool of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong (SAR), People’s Republic of ChinaDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong (SAR), People’s Republic of ChinaABSTRACTAntimicrobial photodynamic therapy (aPDT) is an innovative approach to combat multi-drug resistant bacteria. It is known that cationic Zn(II) phthalocyanines (ZnPc) are effective in mediating aPDT against methicillin-resistant Staphylococcus aureus (MRSA). Here we used ZnPc-based photosensitizer named ZnPcE previously reported by our research group to evaluate its aPDT efficacy against broad spectrum of clinically relevant MRSAs. Remarkably, in vitro anti-MRSA activity was achieved using near-infrared (NIR, >610 nm) light with minimal bactericidal concentrations ranging <0.019–0.156 µM against the panel of MRSAs. ZnPcE was not only significantly (p < .05) more potent than methylene blue, which is a clinically approved photosensitizer but also demonstrated low cytotoxicity against human fibroblasts cell line (Hs-27) and human immortalized keratinocytes cell line (HaCaT). The toxicity was further evaluated on human 3-D skin constructs and found ZnPcE did not manifest in vivo skin irritation at ≤7.8 µM concentration. In the murine MRSA wound model, ZnPcE with PDT group demonstrated > 4 log10 CFU reduction and the value is significantly higher (p < .05) than all test groups except positive control. To conclude, results of present study provide a scientific basis for future clinical evaluation of ZnPcE-PDT on MRSA wound infection.https://www.tandfonline.com/doi/10.1080/22221751.2020.1790305Methicillin-resistantStaphylococcus aureus (MRSA)antimicrobial photodynamic therapy (aPDT)phthalocyaninemurine wound infection modelcytotoxicity |
| spellingShingle | Priyanga Dharmaratne Baiyan Wang Roy C. H. Wong Ben C. L. Chan Kit-Man Lau Mei-Rong Ke Clara B. S. Lau Dennis K. P. Ng Kwok-Pui Fung Margaret Ip Monosubstituted tricationic Zn(II) phthalocyanine enhances antimicrobial photodynamic inactivation (aPDI) of methicillin-resistant Staphylococcus aureus (MRSA) and cytotoxicity evaluation for topical applications: in vitro and in vivo study Emerging Microbes and Infections Methicillin-resistant Staphylococcus aureus (MRSA) antimicrobial photodynamic therapy (aPDT) phthalocyanine murine wound infection model cytotoxicity |
| title | Monosubstituted tricationic Zn(II) phthalocyanine enhances antimicrobial photodynamic inactivation (aPDI) of methicillin-resistant Staphylococcus aureus (MRSA) and cytotoxicity evaluation for topical applications: in vitro and in vivo study |
| title_full | Monosubstituted tricationic Zn(II) phthalocyanine enhances antimicrobial photodynamic inactivation (aPDI) of methicillin-resistant Staphylococcus aureus (MRSA) and cytotoxicity evaluation for topical applications: in vitro and in vivo study |
| title_fullStr | Monosubstituted tricationic Zn(II) phthalocyanine enhances antimicrobial photodynamic inactivation (aPDI) of methicillin-resistant Staphylococcus aureus (MRSA) and cytotoxicity evaluation for topical applications: in vitro and in vivo study |
| title_full_unstemmed | Monosubstituted tricationic Zn(II) phthalocyanine enhances antimicrobial photodynamic inactivation (aPDI) of methicillin-resistant Staphylococcus aureus (MRSA) and cytotoxicity evaluation for topical applications: in vitro and in vivo study |
| title_short | Monosubstituted tricationic Zn(II) phthalocyanine enhances antimicrobial photodynamic inactivation (aPDI) of methicillin-resistant Staphylococcus aureus (MRSA) and cytotoxicity evaluation for topical applications: in vitro and in vivo study |
| title_sort | monosubstituted tricationic zn ii phthalocyanine enhances antimicrobial photodynamic inactivation apdi of methicillin resistant staphylococcus aureus mrsa and cytotoxicity evaluation for topical applications in vitro and in vivo study |
| topic | Methicillin-resistant Staphylococcus aureus (MRSA) antimicrobial photodynamic therapy (aPDT) phthalocyanine murine wound infection model cytotoxicity |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1790305 |
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