Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation

Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation

Short-term germ cell survival and central tissue degeneration limit organoid cultures. Here, testicular organoids (TOs) were generated from two different mouse strains in 3D printed one-layer scaffolds (1LS) at the air-medium interface displaying tubule-like structures and Leydig cell functionality...

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Bibliographic Details
Main Authors: Guillaume Richer, Robin M. Hobbs, Katherine L. Loveland, Ellen Goossens, Yoni Baert
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-12-01
Series:Frontiers in Physiology
Subjects:
organoid
3D printing
testis, spermatogonial stem cells, germline stem cells
tubulogenesis
in vitro spermatogenesis
Online Access:https://www.frontiersin.org/articles/10.3389/fphys.2021.757565/full

Internet

https://www.frontiersin.org/articles/10.3389/fphys.2021.757565/full

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