Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation
Short-term germ cell survival and central tissue degeneration limit organoid cultures. Here, testicular organoids (TOs) were generated from two different mouse strains in 3D printed one-layer scaffolds (1LS) at the air-medium interface displaying tubule-like structures and Leydig cell functionality...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2021.757565/full |
| _version_ | 1819095968366198784 |
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| author | Guillaume Richer Robin M. Hobbs Katherine L. Loveland Katherine L. Loveland Ellen Goossens Yoni Baert |
| author_facet | Guillaume Richer Robin M. Hobbs Katherine L. Loveland Katherine L. Loveland Ellen Goossens Yoni Baert |
| author_sort | Guillaume Richer |
| collection | DOAJ |
| description | Short-term germ cell survival and central tissue degeneration limit organoid cultures. Here, testicular organoids (TOs) were generated from two different mouse strains in 3D printed one-layer scaffolds (1LS) at the air-medium interface displaying tubule-like structures and Leydig cell functionality supporting long-term survival and differentiation of germ cells to the meiotic phase. Chimeric TOs, consisting of a mixture of primary testicular cells and EGFP+ germline stem (GS) cells, were cultured in two-layer scaffolds (2LSs) for better entrapment. They showed an improved spheroidal morphology consisting of one intact tubule-like structure and surrounding interstitium, representing the functional unit of a testis. However, GS cells did not survive long-term culture. Consequently, further optimization of the culture medium is required to enhance the maintenance and differentiation of germ cells. The opportunities TOs offer to manipulate somatic and germ cells are essential for the study of male infertility and the search for potential therapies. |
| first_indexed | 2024-12-21T23:51:44Z |
| format | Article |
| id | doaj.art-f81238d084144c9d9c0419c981db797b |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-21T23:51:44Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-f81238d084144c9d9c0419c981db797b2022-12-21T18:45:55ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2021-12-011210.3389/fphys.2021.757565757565Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface CultivationGuillaume Richer0Robin M. Hobbs1Katherine L. Loveland2Katherine L. Loveland3Ellen Goossens4Yoni Baert5Biology of the Testis Lab, Vrije Universiteit Brussel (VUB), University Medical Campus, Brussels, BelgiumCentre for Reproductive Health, Hudson Institute of Medical Research, Clayton, VIC, AustraliaCentre for Reproductive Health, Hudson Institute of Medical Research, Clayton, VIC, AustraliaDepartment of Molecular and Translational Sciences, School of Clinical Sciences, Monash Medical Centre, Monash University, Clayton, VIC, AustraliaBiology of the Testis Lab, Vrije Universiteit Brussel (VUB), University Medical Campus, Brussels, BelgiumBiology of the Testis Lab, Vrije Universiteit Brussel (VUB), University Medical Campus, Brussels, BelgiumShort-term germ cell survival and central tissue degeneration limit organoid cultures. Here, testicular organoids (TOs) were generated from two different mouse strains in 3D printed one-layer scaffolds (1LS) at the air-medium interface displaying tubule-like structures and Leydig cell functionality supporting long-term survival and differentiation of germ cells to the meiotic phase. Chimeric TOs, consisting of a mixture of primary testicular cells and EGFP+ germline stem (GS) cells, were cultured in two-layer scaffolds (2LSs) for better entrapment. They showed an improved spheroidal morphology consisting of one intact tubule-like structure and surrounding interstitium, representing the functional unit of a testis. However, GS cells did not survive long-term culture. Consequently, further optimization of the culture medium is required to enhance the maintenance and differentiation of germ cells. The opportunities TOs offer to manipulate somatic and germ cells are essential for the study of male infertility and the search for potential therapies.https://www.frontiersin.org/articles/10.3389/fphys.2021.757565/fullorganoid3D printingtestis, spermatogonial stem cells, germline stem cellstubulogenesisin vitro spermatogenesis |
| spellingShingle | Guillaume Richer Robin M. Hobbs Katherine L. Loveland Katherine L. Loveland Ellen Goossens Yoni Baert Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation Frontiers in Physiology organoid 3D printing testis, spermatogonial stem cells, germline stem cells tubulogenesis in vitro spermatogenesis |
| title | Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation |
| title_full | Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation |
| title_fullStr | Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation |
| title_full_unstemmed | Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation |
| title_short | Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation |
| title_sort | long term maintenance and meiotic entry of early germ cells in murine testicular organoids functionalized by 3d printed scaffolds and air medium interface cultivation |
| topic | organoid 3D printing testis, spermatogonial stem cells, germline stem cells tubulogenesis in vitro spermatogenesis |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2021.757565/full |
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