Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation

Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation

Short-term germ cell survival and central tissue degeneration limit organoid cultures. Here, testicular organoids (TOs) were generated from two different mouse strains in 3D printed one-layer scaffolds (1LS) at the air-medium interface displaying tubule-like structures and Leydig cell functionality...

Full description

Bibliographic Details
Main Authors:	Guillaume Richer, Robin M. Hobbs, Katherine L. Loveland, Ellen Goossens, Yoni Baert
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2021-12-01
Series:	Frontiers in Physiology
Subjects:	organoid 3D printing testis, spermatogonial stem cells, germline stem cells tubulogenesis in vitro spermatogenesis
Online Access:	https://www.frontiersin.org/articles/10.3389/fphys.2021.757565/full

Similar Items

Biology and manipulation technologies of male germline stem cells in mammals
by: Seiji Takashima
Published: (2018-10-01)

Investigation Of VASA Gene And Protein Expression In Neonate And Adult Testicular Germ Cells In Mice In Vivo And In Vitro
by: Hossein Azizi, et al.
Published: (2020-06-01)

Generation of a Highly Biomimetic Organoid, Including Vasculature, Resembling the Native Immature Testis Tissue
by: Tat-Chuan Cham, et al.
Published: (2021-07-01)

Effect of Temperature on the Development of Stages of Spermatogenesis and the Functionality of Sertoli Cells In Vitro
by: Areej Jorban, et al.
Published: (2024-02-01)

All eyes on FOXC2
by: Wei Yan, et al.
Published: (2023-08-01)

Single-cell RNA-seq unravels alterations of the human spermatogonial stem cell compartment in patients with impaired spermatogenesis
by: Sara Di Persio, et al.
Published: (2021-09-01)

Gdnf Acts as a Germ Cell-Derived Growth Factor and Regulates the Zebrafish Germ Stem Cell Niche in Autocrine- and Paracrine-Dependent Manners
by: Lucas B. Doretto, et al.
Published: (2022-04-01)

Spermatogonial Stem Cells for In Vitro Spermatogenesis and In Vivo Restoration of Fertility
by: Fahar Ibtisham, et al.
Published: (2020-03-01)

Cellular Therapy via Spermatogonial Stem Cells for Treating Impaired Spermatogenesis, Non-Obstructive Azoospermia
by: Nesma E. Abdelaal, et al.
Published: (2021-07-01)

In vitro spermatogenesis – Optimal culture conditions for testicular cell survival, germ cell differentiation and steroidogenesis in rats
by: Ahmed eReda, et al.
Published: (2014-02-01)

Comparison Of PLZF Gene Expression Between Pluripotent Stem Cells And Testicular Germ Cells
by: Hossein Azizi, et al.
Published: (2020-04-01)

Gestational Exposure to Bisphenol A Affects Testicular Morphology, Germ Cell Associations, and Functions of Spermatogonial Stem Cells in Male Offspring
by: Polash Chandra Karmakar, et al.
Published: (2020-11-01)

Non-neutral clonal selection and its potential role in mammalian germline stem cell dysfunction with advancing age
by: Victor Stolzenbach, et al.
Published: (2022-08-01)

Spermatogonial fate in mice with increased activin A bioactivity and testicular somatic cell tumours
by: Penny A. F. Whiley, et al.
Published: (2023-07-01)

Sperm DNA methylation is predominantly stable in mice offspring born after transplantation of long-term cultured spermatogonial stem cells
by: Joana B. Serrano, et al.
Published: (2023-04-01)

Characterization and Survival of Human Infant Testicular Cells After Direct Xenotransplantation
by: Danyang Wang, et al.
Published: (2022-03-01)

Meiotic Chromosome Synapsis and XY-Body Formation In Vitro
by: Qijing Lei, et al.
Published: (2021-10-01)

Expression profile of spermatogenesis associated genes in male germ cells during postnatal development in mice
by: Jin Seop Ahn, et al.
Published: (2020-12-01)

Germline-competent stem cell in avian species and its application
by: Jae Yong Han, et al.
Published: (2015-06-01)

Perspectivesof spermatogonial stem cellsuse for investigation of spermatogenesis mechanisms and for treatment of male infertility
by: M. V. Polyakova
Published: (2017-02-01)

Co-transplantation of mesenchymal stem cells improves spermatogonial stem cell transplantation efficiency in mice
by: Prashant Kadam, et al.
Published: (2018-11-01)

Core binding factor subunit β plays diverse and essential roles in the male germline
by: Mustika Rahmawati, et al.
Published: (2023-11-01)

Pluripotency Potential of Embryonic Stem Cell-Like Cells Derived from Mouse Testis
by: Hossein Azizi, et al.
Published: (2019-06-01)

Transcriptome Profiling of Developing Testes and First Wave of Spermatogenesis in the Rat
by: Yan Zhang, et al.
Published: (2023-01-01)

Glycolysis-Optimized Conditions Enhance Maintenance of Regenerative Integrity in Mouse Spermatogonial Stem Cells during Long-Term Culture
by: Aileen R. Helsel, et al.
Published: (2017-05-01)

Functions of somatic cells for spermatogenesis in stallions
by: Muhammad Shakeel, et al.
Published: (2022-07-01)

Role of stem cells in fertility preservation: current insights
by: Vermeulen M, et al.
Published: (2019-08-01)

Step by Step about Germ Cells Development in Canine
by: Aline Fernanda de Souza, et al.
Published: (2021-02-01)

Germ Cell Maintenance and Sustained Testosterone and Precursor Hormone Production in Human Prepubertal Testis Organ Culture with Tissues from Boys 7 Years+ under Conditions from Adult Testicular Tissue
by: Neels Lennart Aden, et al.
Published: (2023-01-01)

Bisamide Derivative of Dicarboxylic Acid Contributes to Restoration of Testicular Tissue Function and Influences Spermatogonial Stem Cells in Metabolic Disorders
by: Angelina Pakhomova, et al.
Published: (2020-12-01)

Generation of a dual-color reporter mouse line to monitor spermatogenesis in vivo
by: Yoshinori eMakino, et al.
Published: (2014-07-01)

Establishment of Etv5 gene knockout mice as a recipient model for spermatogonial stem cell transplantation
by: Xianyu Zhang, et al.
Published: (2021-01-01)

Three-Dimensional Culture of Mouse Spermatogonial Stem Cells Using A Decellularised Testicular Scaffold
by: Nasrin Majidi Gharenaz, et al.
Published: (2019-08-01)

Optimizing Immature Testicular Tissue and Cell Transplantation Results: Comparing Transplantation Sites and Scaffolds
by: Alireza Anvari, et al.
Published: (2024-01-01)

Identification of quiescent FOXC2+ spermatogonial stem cells in adult mammals
by: Zhipeng Wang, et al.
Published: (2023-08-01)

Establishment of a <i>Coilia nasus</i> Gonadal Somatic Cell Line Capable of Sperm Induction <i>In Vitro</i>
by: Yuting Kan, et al.
Published: (2022-07-01)

Study on the SHP2-Mediated Mechanism of Promoting Spermatogenesis Induced by Active Compounds of Eucommiae Folium in Mice
by: Hailong Mu, et al.
Published: (2022-03-01)

Isolation and characterization of human spermatogonial stem cells
by: Liu Shixue, et al.
Published: (2011-10-01)

Transcriptome-wide Dynamics of m6A mRNA Methylation During Porcine Spermatogenesis
by: Zidong Liu, et al.
Published: (2023-08-01)

Transcription and chromatin regulation by TAF4b during cellular quiescence of developing prospermatogonia
by: Megan A. Gura, et al.
Published: (2023-10-01)