Multiplexed bead-based assay for the simultaneous quantification of human serum IgG antibodies to tetanus, diphtheria, pertussis toxin, filamentous hemagglutinin, and pertactin

Multiplexed bead-based assay for the simultaneous quantification of human serum IgG antibodies to tetanus, diphtheria, pertussis toxin, filamentous hemagglutinin, and pertactin

BackgroundLuminex bead-based assays offer multiplexing to test antibodies against multiple antigens simultaneously; however, this requires validation using internationally certified reference standards. Therefore, there is an urgent need to characterize existing reference standards for the standardi...

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Bibliographic Details
Main Authors: Vishal Rathod, Laxmikant Kadam, Manish Gautam, Prabhu Dasu Gumma, Kevin Marke, Cathy Asokanathan, Alex Douglas-Bardsley, Laura Hassell, Sachin Bhandare, Sumit Gupta, Sameer Parekh, Pramod Pujari, Harish Rao, Hitt Sharma, Umesh Shaligram, Sunil Gairola
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-06-01
Series:Frontiers in Immunology
Subjects:
diphtheria
enzyme-linked immunosorbent assay (ELISA)
Luminex (xMAP) method
multiplex immunoassay (MIA)
pertussis
tetanus
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2023.1190404/full

Internet

https://www.frontiersin.org/articles/10.3389/fimmu.2023.1190404/full

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