Cardiac <i>Hamp</i> mRNA Is Predominantly Expressed in the Right Atrium and Does Not Respond to Iron

Hepcidin is a liver-derived hormone that controls systemic iron traffic. It is also expressed in the heart, where it acts locally. We utilized cell and mouse models to study the regulation, expression, and function of cardiac hepcidin. Hepcidin-encoding <i>Hamp</i> mRNA was induced upon...

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Main Authors: Maria Bigorra Mir, Edouard Charlebois, Sofiya Tsyplenkova, Carine Fillebeen, Kostas Pantopoulos
Format: Article
Language:English
Published: MDPI AG 2023-03-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/24/6/5163
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author Maria Bigorra Mir
Edouard Charlebois
Sofiya Tsyplenkova
Carine Fillebeen
Kostas Pantopoulos
author_facet Maria Bigorra Mir
Edouard Charlebois
Sofiya Tsyplenkova
Carine Fillebeen
Kostas Pantopoulos
author_sort Maria Bigorra Mir
collection DOAJ
description Hepcidin is a liver-derived hormone that controls systemic iron traffic. It is also expressed in the heart, where it acts locally. We utilized cell and mouse models to study the regulation, expression, and function of cardiac hepcidin. Hepcidin-encoding <i>Hamp</i> mRNA was induced upon differentiation of C2C12 cells to a cardiomyocyte-like phenotype and was not further stimulated by BMP6, BMP2, or IL-6, the major inducers of hepatic hepcidin. The mRNAs encoding hepcidin and its upstream regulator hemojuvelin (Hjv) are primarily expressed in the atria of the heart, with ~20-fold higher <i>Hamp</i> mRNA levels in the right vs. left atrium and negligible expression in the ventricles and apex. Hjv<sup>−/−</sup> mice, a model of hemochromatosis due to suppression of liver hepcidin, exhibit only modest cardiac <i>Hamp</i> deficiency and minor cardiac dysfunction. Dietary iron manipulations did not significantly affect cardiac <i>Hamp</i> mRNA in the atria of wild-type or Hjv<sup>−/−</sup> mice. Two weeks following myocardial infarction, <i>Hamp</i> was robustly induced in the liver and heart apex but not atria, possibly in response to inflammation. We conclude that cardiac <i>Hamp</i> is predominantly expressed in the right atrium and is partially regulated by Hjv; however, it does not respond to iron and other inducers of hepatic hepcidin.
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spelling doaj.art-f825ce2bd1fb4badb10877364a3f94612023-11-17T11:30:02ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246516310.3390/ijms24065163Cardiac <i>Hamp</i> mRNA Is Predominantly Expressed in the Right Atrium and Does Not Respond to IronMaria Bigorra Mir0Edouard Charlebois1Sofiya Tsyplenkova2Carine Fillebeen3Kostas Pantopoulos4Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, QC H3T 1E2, CanadaLady Davis Institute for Medical Research, Jewish General Hospital, Montreal, QC H3T 1E2, CanadaLady Davis Institute for Medical Research, Jewish General Hospital, Montreal, QC H3T 1E2, CanadaLady Davis Institute for Medical Research, Jewish General Hospital, Montreal, QC H3T 1E2, CanadaLady Davis Institute for Medical Research, Jewish General Hospital, Montreal, QC H3T 1E2, CanadaHepcidin is a liver-derived hormone that controls systemic iron traffic. It is also expressed in the heart, where it acts locally. We utilized cell and mouse models to study the regulation, expression, and function of cardiac hepcidin. Hepcidin-encoding <i>Hamp</i> mRNA was induced upon differentiation of C2C12 cells to a cardiomyocyte-like phenotype and was not further stimulated by BMP6, BMP2, or IL-6, the major inducers of hepatic hepcidin. The mRNAs encoding hepcidin and its upstream regulator hemojuvelin (Hjv) are primarily expressed in the atria of the heart, with ~20-fold higher <i>Hamp</i> mRNA levels in the right vs. left atrium and negligible expression in the ventricles and apex. Hjv<sup>−/−</sup> mice, a model of hemochromatosis due to suppression of liver hepcidin, exhibit only modest cardiac <i>Hamp</i> deficiency and minor cardiac dysfunction. Dietary iron manipulations did not significantly affect cardiac <i>Hamp</i> mRNA in the atria of wild-type or Hjv<sup>−/−</sup> mice. Two weeks following myocardial infarction, <i>Hamp</i> was robustly induced in the liver and heart apex but not atria, possibly in response to inflammation. We conclude that cardiac <i>Hamp</i> is predominantly expressed in the right atrium and is partially regulated by Hjv; however, it does not respond to iron and other inducers of hepatic hepcidin.https://www.mdpi.com/1422-0067/24/6/5163hepcidinhemojuvelinferroportiniron metabolismatriacardiomyocytes
spellingShingle Maria Bigorra Mir
Edouard Charlebois
Sofiya Tsyplenkova
Carine Fillebeen
Kostas Pantopoulos
Cardiac <i>Hamp</i> mRNA Is Predominantly Expressed in the Right Atrium and Does Not Respond to Iron
International Journal of Molecular Sciences
hepcidin
hemojuvelin
ferroportin
iron metabolism
atria
cardiomyocytes
title Cardiac <i>Hamp</i> mRNA Is Predominantly Expressed in the Right Atrium and Does Not Respond to Iron
title_full Cardiac <i>Hamp</i> mRNA Is Predominantly Expressed in the Right Atrium and Does Not Respond to Iron
title_fullStr Cardiac <i>Hamp</i> mRNA Is Predominantly Expressed in the Right Atrium and Does Not Respond to Iron
title_full_unstemmed Cardiac <i>Hamp</i> mRNA Is Predominantly Expressed in the Right Atrium and Does Not Respond to Iron
title_short Cardiac <i>Hamp</i> mRNA Is Predominantly Expressed in the Right Atrium and Does Not Respond to Iron
title_sort cardiac i hamp i mrna is predominantly expressed in the right atrium and does not respond to iron
topic hepcidin
hemojuvelin
ferroportin
iron metabolism
atria
cardiomyocytes
url https://www.mdpi.com/1422-0067/24/6/5163
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AT sofiyatsyplenkova cardiacihampimrnaispredominantlyexpressedintherightatriumanddoesnotrespondtoiron
AT carinefillebeen cardiacihampimrnaispredominantlyexpressedintherightatriumanddoesnotrespondtoiron
AT kostaspantopoulos cardiacihampimrnaispredominantlyexpressedintherightatriumanddoesnotrespondtoiron