Improved base editing and functional screening in Leishmania via co-expression of the AsCas12a ultra variant, a T7 RNA polymerase, and a cytosine base editor

Improved base editing and functional screening in Leishmania via co-expression of the AsCas12a ultra variant, a T7 RNA polymerase, and a cytosine base editor

The ability to analyze the function of all genes in a genome is highly desirable, yet challenging in Leishmania due to a repetitive genome, limited DNA repair mechanisms, and lack of RNA interference in most species. While our introduction of a cytosine base editor (CBE) demonstrated potential to ov...

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Bibliographic Details
Main Authors: Nicole Herrmann May, Anh Cao, Annika Schmid, Fabian Link, Jorge Arias-del-Angel, Elisabeth Meiser, Tom Beneke
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2025-02-01
Series:eLife
Subjects:
Leishmania
cytosine base editing
CRISPR
functional screening
Cas12a
loss-of-function
Online Access:https://elifesciences.org/articles/97437

Internet

https://elifesciences.org/articles/97437

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