Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties
Abstract Background Pru p 1 is a major allergen in peach and nectarine, and the different content in varieties may affect the degree of allergic reactions. This study aimed to quantify Pru p 1 levels in representative peach varieties and select hypoallergenic Pru p 1 varieties. Methods To obtain mon...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-05-01
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| Series: | Clinical and Translational Allergy |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/clt2.12034 |
| _version_ | 1818903450046431232 |
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| author | Jing Jin Kexin Gan Lan Zhao Huijuan Jia Yifan Zhu Xiongwei Li Zhaowei Yang Zhengwen Ye Ke Cao Zhiqiang Wang Mingliang Yu Yuyan Zhang Zhisheng Ma Hangkong Liu Pere Arús Jaap H. Akkerdaas Zhongshan Gao Ronald van Ree |
| author_facet | Jing Jin Kexin Gan Lan Zhao Huijuan Jia Yifan Zhu Xiongwei Li Zhaowei Yang Zhengwen Ye Ke Cao Zhiqiang Wang Mingliang Yu Yuyan Zhang Zhisheng Ma Hangkong Liu Pere Arús Jaap H. Akkerdaas Zhongshan Gao Ronald van Ree |
| author_sort | Jing Jin |
| collection | DOAJ |
| description | Abstract Background Pru p 1 is a major allergen in peach and nectarine, and the different content in varieties may affect the degree of allergic reactions. This study aimed to quantify Pru p 1 levels in representative peach varieties and select hypoallergenic Pru p 1 varieties. Methods To obtain monoclonal and polyclonal antibodies, mice and rabbits, respectively, were immunized with recombinant Pru p 1.01 and Pru p 1.02. The Pru p 1 levels in fruits from 83 representative peach varieties was quantified by sandwich enzyme‐linked immunosorbent assay (sELISA). nPru p 1 was obtained through specific monoclonal antibody affinity purification and confirmed by Western blot and mass spectrometry. The variable Pru p 1 content of selected varieties was evaluated by Western blot and the expression level of encoding Pru p 1 genes by quantitative polymerase chain reaction. Results A sELISA method with monoclonal and polyclonal antibodies was built for quantifying Pru p 1 levels in peach. Pru p 1 was mainly concentrated in the peel (0.20–73.44 μg/g, fresh weight), being very low in the pulp (0.05–9.62 μg/g) and not detected in wild peach. For the 78 peach and nectarine varieties, Pru p 1 content varied widely from 0.12 to 6.45 μg/g in whole fruit. We verified that natural Pru p 1 is composed of 1.01 and 1.02 isoallergens, and the Pru p 1 expression level and Pru p 1 band intensity in the immunoblots were in agreement with protein quantity determined by ELISA for some tested varieties. In some cases, the reduced levels of Pru p 1 did not coincide with low Pru p 3 in the same variety in whole fruit, while some ancient wild peach and nectarines contained low levels of both allergens, and late‐ripening yellow flesh varieties were usually highly allergenic. Conclusion Pru p 1 content is generally low in peach compared to Pru p 3. Several hypoallergenic Pru p 1 and Pru p 3 varieties, “Zi Xue Tao,” “Wu Yue Xian,” and “May Fire,” were identified, which could be useful in trials for peach allergy patients. |
| first_indexed | 2024-12-19T20:51:44Z |
| format | Article |
| id | doaj.art-f8485b7803824f0289c63f38d9cdc9a5 |
| institution | Directory Open Access Journal |
| issn | 2045-7022 |
| language | English |
| last_indexed | 2024-12-19T20:51:44Z |
| publishDate | 2021-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Translational Allergy |
| spelling | doaj.art-f8485b7803824f0289c63f38d9cdc9a52022-12-21T20:06:06ZengWileyClinical and Translational Allergy2045-70222021-05-01113n/an/a10.1002/clt2.12034Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varietiesJing Jin0Kexin Gan1Lan Zhao2Huijuan Jia3Yifan Zhu4Xiongwei Li5Zhaowei Yang6Zhengwen Ye7Ke Cao8Zhiqiang Wang9Mingliang Yu10Yuyan Zhang11Zhisheng Ma12Hangkong Liu13Pere Arús14Jaap H. Akkerdaas15Zhongshan Gao16Ronald van Ree17Allergy Research Center Zhejiang University Hangzhou ChinaCollege of Agriculture and Biotechnology Zhejiang University Hangzhou ChinaAllergy Research Center Zhejiang University Hangzhou ChinaCollege of Agriculture and Biotechnology Zhejiang University Hangzhou ChinaCollege of Agriculture and Biotechnology Zhejiang University Hangzhou ChinaForest & Fruit Tree Institute Shanghai Academy of Agricultural Sciences Shanghai ChinaState Key Laboratory of Respiratory Disease The First Affiliated Hospital of Guangzhou Medical University Guangzhou ChinaForest & Fruit Tree Institute Shanghai Academy of Agricultural Sciences Shanghai ChinaZhengzhou Fruit Research Institute China Academy of Agricultural Sciences Zhengzhou ChinaZhengzhou Fruit Research Institute China Academy of Agricultural Sciences Zhengzhou ChinaFruit Tree Institute Jiangsu Academy of Agricultural Sciences Nanjing ChinaFruit Tree Institute Jiangsu Academy of Agricultural Sciences Nanjing ChinaShijiazhuang Pomology Institute Hebei Academy of Agriculture and Forestry Sciences Shijiazhuang Hebei ChinaCollege of Horticulture Northwest A&F University Yangling Shaanxi ChinaIRTA Centre de Recerca en Agrigenòmica CSIC‐IRTA‐UAB‐UB Campus UAB – Edifici CRAG Barcelona SpainDepartments of Experimental Immunology and Otorhinolaryngology Amsterdam UMC University of Amsterdam Amsterdam The NetherlandsAllergy Research Center Zhejiang University Hangzhou ChinaDepartments of Experimental Immunology and Otorhinolaryngology Amsterdam UMC University of Amsterdam Amsterdam The NetherlandsAbstract Background Pru p 1 is a major allergen in peach and nectarine, and the different content in varieties may affect the degree of allergic reactions. This study aimed to quantify Pru p 1 levels in representative peach varieties and select hypoallergenic Pru p 1 varieties. Methods To obtain monoclonal and polyclonal antibodies, mice and rabbits, respectively, were immunized with recombinant Pru p 1.01 and Pru p 1.02. The Pru p 1 levels in fruits from 83 representative peach varieties was quantified by sandwich enzyme‐linked immunosorbent assay (sELISA). nPru p 1 was obtained through specific monoclonal antibody affinity purification and confirmed by Western blot and mass spectrometry. The variable Pru p 1 content of selected varieties was evaluated by Western blot and the expression level of encoding Pru p 1 genes by quantitative polymerase chain reaction. Results A sELISA method with monoclonal and polyclonal antibodies was built for quantifying Pru p 1 levels in peach. Pru p 1 was mainly concentrated in the peel (0.20–73.44 μg/g, fresh weight), being very low in the pulp (0.05–9.62 μg/g) and not detected in wild peach. For the 78 peach and nectarine varieties, Pru p 1 content varied widely from 0.12 to 6.45 μg/g in whole fruit. We verified that natural Pru p 1 is composed of 1.01 and 1.02 isoallergens, and the Pru p 1 expression level and Pru p 1 band intensity in the immunoblots were in agreement with protein quantity determined by ELISA for some tested varieties. In some cases, the reduced levels of Pru p 1 did not coincide with low Pru p 3 in the same variety in whole fruit, while some ancient wild peach and nectarines contained low levels of both allergens, and late‐ripening yellow flesh varieties were usually highly allergenic. Conclusion Pru p 1 content is generally low in peach compared to Pru p 3. Several hypoallergenic Pru p 1 and Pru p 3 varieties, “Zi Xue Tao,” “Wu Yue Xian,” and “May Fire,” were identified, which could be useful in trials for peach allergy patients.https://doi.org/10.1002/clt2.12034酶联免疫吸附测试低致敏品种桃过敏蛋白定量Pru p 1 |
| spellingShingle | Jing Jin Kexin Gan Lan Zhao Huijuan Jia Yifan Zhu Xiongwei Li Zhaowei Yang Zhengwen Ye Ke Cao Zhiqiang Wang Mingliang Yu Yuyan Zhang Zhisheng Ma Hangkong Liu Pere Arús Jaap H. Akkerdaas Zhongshan Gao Ronald van Ree Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties Clinical and Translational Allergy 酶联免疫吸附测试 低致敏品种 桃过敏 蛋白定量 Pru p 1 |
| title | Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties |
| title_full | Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties |
| title_fullStr | Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties |
| title_full_unstemmed | Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties |
| title_short | Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties |
| title_sort | peach allergen pru p 1 content is generally low in fruit but with large variation in different varieties |
| topic | 酶联免疫吸附测试 低致敏品种 桃过敏 蛋白定量 Pru p 1 |
| url | https://doi.org/10.1002/clt2.12034 |
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