Production of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks

Production of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks

Abstract Background Shake flasks are widely used during the development of bioprocesses for recombinant proteins. Cultures of recombinant Escherichia coli with orbital mixing (OM) have an oxygen limitation negatively affecting biomass growth and recombinant-protein production. With the aim to improv...

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Bibliographic Details
Main Authors: Norma A. Valdez-Cruz, Greta I. Reynoso-Cereceda, Saumel Pérez-Rodriguez, Sara Restrepo-Pineda, Jesus González-Santana, Alejandro Olvera, Guadalupe Zavala, Alejandro Alagón, Mauricio A. Trujillo-Roldán
Format: Article
Language:English
Published: BMC 2017-07-01
Series:Microbial Cell Factories
Subjects:
Resonant acoustic mixing
Orbital mixing
Shake flask
Recombinant protein
Inclusion bodies
Escherichia coli
Online Access:http://link.springer.com/article/10.1186/s12934-017-0746-1

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http://link.springer.com/article/10.1186/s12934-017-0746-1

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