Determination of isotretinoin in human plasma: Application to pharmacokinetic study
A simple, rapid, sensitive and accurate HPLC–UV detection method was developed and validated for the determination of isotretinoin in human plasma. The method involves protein precipitation of plasma by acetonitrile using nifedipine as the internal standard (IS). Chromatographic separation was achie...
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| Format: | Article |
| Language: | English |
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Faculty of Pharmacy, Cairo University
2012-12-01
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| Series: | Bulletin of Faculty of Pharmacy Cairo University |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110093112000348 |
| _version_ | 1797967139422863360 |
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| author | Nisreen F. Abo-Talib Marwa H. Tammam Essam E. Hassan |
| author_facet | Nisreen F. Abo-Talib Marwa H. Tammam Essam E. Hassan |
| author_sort | Nisreen F. Abo-Talib |
| collection | DOAJ |
| description | A simple, rapid, sensitive and accurate HPLC–UV detection method was developed and validated for the determination of isotretinoin in human plasma. The method involves protein precipitation of plasma by acetonitrile using nifedipine as the internal standard (IS). Chromatographic separation was achieved on Agilent C18 column using an acetonitrile: aqueous 0.5% glacial acetic acid (70:30, v/v) mobile phase. Isotretinoin in the eluent was monitored at 350 nm. Inter-assay of the coefficient of variations (CV%) over the range of plasma calibration curves (0.02–0.60 μg/ml) ranged from 2.32% to 6.49% with good correlation coefficient (R2 = 0.999). The average recovery of the extraction procedure was 99.128%. The LLOD and LLOQ are 0.007 and 0.02 μg/ml, respectively. This method was applied for pharmacokinetic study and the results indicated that this method is suitable for pharmacokinetic and bioavailability studies. |
| first_indexed | 2024-04-11T02:26:29Z |
| format | Article |
| id | doaj.art-f8a1a017ad3e46238e77b1563954a850 |
| institution | Directory Open Access Journal |
| issn | 1110-0931 |
| language | English |
| last_indexed | 2024-04-11T02:26:29Z |
| publishDate | 2012-12-01 |
| publisher | Faculty of Pharmacy, Cairo University |
| record_format | Article |
| series | Bulletin of Faculty of Pharmacy Cairo University |
| spelling | doaj.art-f8a1a017ad3e46238e77b1563954a8502023-01-02T22:47:03ZengFaculty of Pharmacy, Cairo UniversityBulletin of Faculty of Pharmacy Cairo University1110-09312012-12-0150212713210.1016/j.bfopcu.2012.09.001Determination of isotretinoin in human plasma: Application to pharmacokinetic studyNisreen F. Abo-Talib0Marwa H. Tammam1Essam E. Hassan2Drug Bioavailability Center, National Organization for Drug Control and Research (NODCAR), P.O. Box 29 Cairo, EgyptDrug Bioavailability Center, National Organization for Drug Control and Research (NODCAR), P.O. Box 29 Cairo, EgyptDrug Bioavailability Center, National Organization for Drug Control and Research (NODCAR), P.O. Box 29 Cairo, EgyptA simple, rapid, sensitive and accurate HPLC–UV detection method was developed and validated for the determination of isotretinoin in human plasma. The method involves protein precipitation of plasma by acetonitrile using nifedipine as the internal standard (IS). Chromatographic separation was achieved on Agilent C18 column using an acetonitrile: aqueous 0.5% glacial acetic acid (70:30, v/v) mobile phase. Isotretinoin in the eluent was monitored at 350 nm. Inter-assay of the coefficient of variations (CV%) over the range of plasma calibration curves (0.02–0.60 μg/ml) ranged from 2.32% to 6.49% with good correlation coefficient (R2 = 0.999). The average recovery of the extraction procedure was 99.128%. The LLOD and LLOQ are 0.007 and 0.02 μg/ml, respectively. This method was applied for pharmacokinetic study and the results indicated that this method is suitable for pharmacokinetic and bioavailability studies.http://www.sciencedirect.com/science/article/pii/S1110093112000348IsotretinoinNifedipineHPLCPharmacokinetics and plasma |
| spellingShingle | Nisreen F. Abo-Talib Marwa H. Tammam Essam E. Hassan Determination of isotretinoin in human plasma: Application to pharmacokinetic study Bulletin of Faculty of Pharmacy Cairo University Isotretinoin Nifedipine HPLC Pharmacokinetics and plasma |
| title | Determination of isotretinoin in human plasma: Application to pharmacokinetic study |
| title_full | Determination of isotretinoin in human plasma: Application to pharmacokinetic study |
| title_fullStr | Determination of isotretinoin in human plasma: Application to pharmacokinetic study |
| title_full_unstemmed | Determination of isotretinoin in human plasma: Application to pharmacokinetic study |
| title_short | Determination of isotretinoin in human plasma: Application to pharmacokinetic study |
| title_sort | determination of isotretinoin in human plasma application to pharmacokinetic study |
| topic | Isotretinoin Nifedipine HPLC Pharmacokinetics and plasma |
| url | http://www.sciencedirect.com/science/article/pii/S1110093112000348 |
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