Comparison of multiplex real-time PCR and ergosterol assays in quantifying Heterobasidion annosum in planta

Comparison of multiplex real-time PCR and ergosterol assays in quantifying Heterobasidion annosum in planta

A quantitative multiplex real-time PCR procedure was developed to monitor the dynamics in Norway spruce-Heterobasidion annosum pathosystem. The assay reliably detected down to 1 pg of H. annosum DNA and 1 ng of host DNA in multiplex conditions. As a comparative method for quantifying fungal coloniza...

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Bibliographic Details
Main Authors: A.M. Hietala, M. Eikenes, M. Kvaalen, H. Solheim, C.G. Fossdal
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2002-12-01
Series:Plant Protection Science
Subjects:
heterobasidion
norway spruce
infection
quantification
Online Access:https://pps.agriculturejournals.cz/artkey/pps-200211-0047_comparison-of-multiplex-real-time-pcr-and-ergosterol-assays-in-quantifying-heterobasidion-annosum-in-planta.php

Internet

https://pps.agriculturejournals.cz/artkey/pps-200211-0047_comparison-of-multiplex-real-time-pcr-and-ergosterol-assays-in-quantifying-heterobasidion-annosum-in-planta.php

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