Paraspinal Muscle Health is Related to Fibrogenic, Adipogenic, and Myogenic Gene Expression in Patients with Lumbar Spine Pathology
Abstract Background Lumbar spine pathology is a common feature of lower back and/or lower extremity pain and is associated with observable degenerative changes in the lumbar paraspinal muscles that are associated with poor clinical prognosis. Despite the commonly observed phenotype of muscle degener...
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | BMC Musculoskeletal Disorders |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12891-022-05572-7 |
| _version_ | 1818249798519619584 |
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| author | Brad Anderson Angel Ordaz Vinko Zlomislic R. Todd Allen Steven R. Garfin Regula Schuepbach Mazda Farshad Simon Schenk Samuel R. Ward Bahar Shahidi |
| author_facet | Brad Anderson Angel Ordaz Vinko Zlomislic R. Todd Allen Steven R. Garfin Regula Schuepbach Mazda Farshad Simon Schenk Samuel R. Ward Bahar Shahidi |
| author_sort | Brad Anderson |
| collection | DOAJ |
| description | Abstract Background Lumbar spine pathology is a common feature of lower back and/or lower extremity pain and is associated with observable degenerative changes in the lumbar paraspinal muscles that are associated with poor clinical prognosis. Despite the commonly observed phenotype of muscle degeneration in this patient population, its underlying molecular mechanisms are not well understood. The aim of this study was to investigate the relationships between groups of genes within the atrophic, myogenic, fibrogenic, adipogenic, and inflammatory pathways and multifidus muscle health in individuals undergoing surgery for lumbar spine pathology. Methods Multifidus muscle biopsies were obtained from patients (n = 59) undergoing surgery for lumbar spine pathology to analyze 42 genes from relevant adipogenic/metabolic, atrophic, fibrogenic, inflammatory, and myogenic gene pathways using quantitative polymerase chain reaction. Multifidus muscle morphology was examined preoperatively in these patients at the level and side of biopsy using T2-weighted magnetic resonance imaging to determine whole muscle compartment area, lean muscle area, fat cross-sectional areas, and proportion of fat within the muscle compartment. These measures were used to investigate the relationships between gene expression patterns and muscle size and quality. Results Relationships between gene expression and imaging revealed significant associations between decreased expression of adipogenic/metabolic gene (PPARD), increased expression of fibrogenic gene (COL3A1), and lower fat fraction on MRI (r = -0.346, p = 0.018, and r = 0.386, p = 0.047 respectively). Decreased expression of myogenic gene (mTOR) was related to greater lean muscle cross-sectional area (r = 0.388, p = 0.045). Conclusion Fibrogenic and adipogenic/metabolic genes were related to pre-operative muscle quality, and myogenic genes were related to pre-operative muscle size. These findings provide insight into molecular pathways associated with muscle health in the presence of lumbar spine pathology, establishing a foundation for future research that addresses how these changes impact outcomes in this patient population. |
| first_indexed | 2024-12-12T15:42:13Z |
| format | Article |
| id | doaj.art-f8d34660119f4464aedf0aca3c670ae9 |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-12-12T15:42:13Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-f8d34660119f4464aedf0aca3c670ae92022-12-22T00:19:52ZengBMCBMC Musculoskeletal Disorders1471-24742022-06-0123111110.1186/s12891-022-05572-7Paraspinal Muscle Health is Related to Fibrogenic, Adipogenic, and Myogenic Gene Expression in Patients with Lumbar Spine PathologyBrad Anderson0Angel Ordaz1Vinko Zlomislic2R. Todd Allen3Steven R. Garfin4Regula Schuepbach5Mazda Farshad6Simon Schenk7Samuel R. Ward8Bahar Shahidi9Department of Orthopaedic Surgery, University of California San DiegoDepartment of Orthopaedic Surgery, University of California San DiegoDepartment of Orthopaedic Surgery, University of California San DiegoDepartment of Orthopaedic Surgery, University of California San DiegoDepartment of Orthopaedic Surgery, University of California San DiegoDepartment of Orthopaedics, Balgrist University Hospital, University of ZurichDepartment of Orthopaedics, Balgrist University Hospital, University of ZurichDepartment of Orthopaedic Surgery, University of California San DiegoDepartment of Orthopaedic Surgery, University of California San DiegoDepartment of Orthopaedic Surgery, University of California San DiegoAbstract Background Lumbar spine pathology is a common feature of lower back and/or lower extremity pain and is associated with observable degenerative changes in the lumbar paraspinal muscles that are associated with poor clinical prognosis. Despite the commonly observed phenotype of muscle degeneration in this patient population, its underlying molecular mechanisms are not well understood. The aim of this study was to investigate the relationships between groups of genes within the atrophic, myogenic, fibrogenic, adipogenic, and inflammatory pathways and multifidus muscle health in individuals undergoing surgery for lumbar spine pathology. Methods Multifidus muscle biopsies were obtained from patients (n = 59) undergoing surgery for lumbar spine pathology to analyze 42 genes from relevant adipogenic/metabolic, atrophic, fibrogenic, inflammatory, and myogenic gene pathways using quantitative polymerase chain reaction. Multifidus muscle morphology was examined preoperatively in these patients at the level and side of biopsy using T2-weighted magnetic resonance imaging to determine whole muscle compartment area, lean muscle area, fat cross-sectional areas, and proportion of fat within the muscle compartment. These measures were used to investigate the relationships between gene expression patterns and muscle size and quality. Results Relationships between gene expression and imaging revealed significant associations between decreased expression of adipogenic/metabolic gene (PPARD), increased expression of fibrogenic gene (COL3A1), and lower fat fraction on MRI (r = -0.346, p = 0.018, and r = 0.386, p = 0.047 respectively). Decreased expression of myogenic gene (mTOR) was related to greater lean muscle cross-sectional area (r = 0.388, p = 0.045). Conclusion Fibrogenic and adipogenic/metabolic genes were related to pre-operative muscle quality, and myogenic genes were related to pre-operative muscle size. These findings provide insight into molecular pathways associated with muscle health in the presence of lumbar spine pathology, establishing a foundation for future research that addresses how these changes impact outcomes in this patient population.https://doi.org/10.1186/s12891-022-05572-7Low back painSkeletal muscleMultifidusDegenerationAtrophyFatty infiltration |
| spellingShingle | Brad Anderson Angel Ordaz Vinko Zlomislic R. Todd Allen Steven R. Garfin Regula Schuepbach Mazda Farshad Simon Schenk Samuel R. Ward Bahar Shahidi Paraspinal Muscle Health is Related to Fibrogenic, Adipogenic, and Myogenic Gene Expression in Patients with Lumbar Spine Pathology BMC Musculoskeletal Disorders Low back pain Skeletal muscle Multifidus Degeneration Atrophy Fatty infiltration |
| title | Paraspinal Muscle Health is Related to Fibrogenic, Adipogenic, and Myogenic Gene Expression in Patients with Lumbar Spine Pathology |
| title_full | Paraspinal Muscle Health is Related to Fibrogenic, Adipogenic, and Myogenic Gene Expression in Patients with Lumbar Spine Pathology |
| title_fullStr | Paraspinal Muscle Health is Related to Fibrogenic, Adipogenic, and Myogenic Gene Expression in Patients with Lumbar Spine Pathology |
| title_full_unstemmed | Paraspinal Muscle Health is Related to Fibrogenic, Adipogenic, and Myogenic Gene Expression in Patients with Lumbar Spine Pathology |
| title_short | Paraspinal Muscle Health is Related to Fibrogenic, Adipogenic, and Myogenic Gene Expression in Patients with Lumbar Spine Pathology |
| title_sort | paraspinal muscle health is related to fibrogenic adipogenic and myogenic gene expression in patients with lumbar spine pathology |
| topic | Low back pain Skeletal muscle Multifidus Degeneration Atrophy Fatty infiltration |
| url | https://doi.org/10.1186/s12891-022-05572-7 |
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