Quality control and statistical evaluation of combinatorial DNA libraries using nanopore sequencing

Protein engineering and synthetic biology applications increasingly rely on the assembly of modular libraries composed of thousands of different combinations of DNA building blocks. At present, the validation of such libraries is performed by Sanger sequencing analysis on a small subset of clones on...

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Bibliographic Details
Main Authors: Cédric Lood, Hans Gerstmans, Yves Briers, Vera van Noort, Rob Lavigne
Format: Article
Language:English
Published: Taylor & Francis Group 2020-11-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/btn-2020-0060