Using CRISPR/Cas9 for Large Fragment Deletions in Saccharomyces cerevisiae
CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9) systems have emerged as a powerful tool for genome editing in many organisms. The wide use of CRISPR/Cas9 systems may be due to the fact that these systems contain a simple guide RNA (sgRNA) that is r...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2017-07-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=2415&type=0 |