CRISPR-Guided Proximity Labeling of RNA–Protein Interactions

CRISPR-Guided Proximity Labeling of RNA–Protein Interactions

Proximity labeling employs modified biotin ligases or peroxidases that produce reactive radicals to covalently label proximate proteins with biotin in living cells. The resulting biotinylated proteins can then be isolated and identified. A combination of programmable DNA targeting and proximity labe...

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Bibliographic Details
Main Authors: Mingxing Lu, Zuowei Wang, Yixiu Wang, Bingbing Ren
Format: Article
Language:English
Published: MDPI AG 2022-08-01
Series:Genes
Subjects:
CRISPR-Cas13
bioID/APEX
proximity labeling
RNA elements
RNA–protein interaction
Online Access:https://www.mdpi.com/2073-4425/13/9/1549

Internet

https://www.mdpi.com/2073-4425/13/9/1549

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