Culture of glioblastoma U87 stem-like cells and identification of its metabolic phenotype and tumorigenic ability

Objective To cultivate glioblastoma U87 stem-like cells (SLCs) and to detect the level of stemness biomarkers, mitochondrial respiratory capacity and the capacity of in vivo tumorigenesis. Methods B-27, growth factors EGF and bFGF was added into DMEM/F-12 culture in serum-free stem cell culture medi...

Full description

Bibliographic Details
Main Author: QIU Jiaxing, LIU Yuhan, GUO Hongjiang, ZHANG Diya, WANG Yucheng, JU Rui, GUO Lei
Format: Article
Language:zho
Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. 2024-01-01
Series:Jichu yixue yu linchuang
Subjects:
Online Access:http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2024-44-1-16.pdf