Culture of glioblastoma U87 stem-like cells and identification of its metabolic phenotype and tumorigenic ability
Objective To cultivate glioblastoma U87 stem-like cells (SLCs) and to detect the level of stemness biomarkers, mitochondrial respiratory capacity and the capacity of in vivo tumorigenesis. Methods B-27, growth factors EGF and bFGF was added into DMEM/F-12 culture in serum-free stem cell culture medi...
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| Формат: | Стаття |
| Мова: | zho |
| Опубліковано: |
Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2024-01-01
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| Серія: | Jichu yixue yu linchuang |
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| Онлайн доступ: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2024-44-1-16.pdf |