High-pressure processing-induced transcriptome response during recovery of Listeria monocytogenes
Abstract Background High-pressure processing (HPP) is a commonly used technique in the food industry to inactivate pathogens, including L. monocytogenes. It has been shown that L. monocytogenes is able to recover from HPP injuries and can start to grow again during long-term cold storage. To date, t...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-02-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-021-07407-6 |
| _version_ | 1818417480794636288 |
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| author | Ilhan Cem Duru Florentina Ionela Bucur Margarita Andreevskaya Bahareh Nikparvar Anne Ylinen Leontina Grigore-Gurgu Tone Mari Rode Peter Crauwels Pia Laine Lars Paulin Trond Løvdal Christian U. Riedel Nadav Bar Daniela Borda Anca Ioana Nicolau Petri Auvinen |
| author_facet | Ilhan Cem Duru Florentina Ionela Bucur Margarita Andreevskaya Bahareh Nikparvar Anne Ylinen Leontina Grigore-Gurgu Tone Mari Rode Peter Crauwels Pia Laine Lars Paulin Trond Løvdal Christian U. Riedel Nadav Bar Daniela Borda Anca Ioana Nicolau Petri Auvinen |
| author_sort | Ilhan Cem Duru |
| collection | DOAJ |
| description | Abstract Background High-pressure processing (HPP) is a commonly used technique in the food industry to inactivate pathogens, including L. monocytogenes. It has been shown that L. monocytogenes is able to recover from HPP injuries and can start to grow again during long-term cold storage. To date, the gene expression profiling of L. monocytogenes during HPP damage recovery at cooling temperature has not been studied. In order identify key genes that play a role in recovery of the damage caused by HPP treatment, we performed RNA-sequencing (RNA-seq) for two L. monocytogenes strains (barotolerant RO15 and barosensitive ScottA) at nine selected time points (up to 48 h) after treatment with two pressure levels (200 and 400 MPa). Results The results showed that a general stress response was activated by SigB after HPP treatment. In addition, the phosphotransferase system (PTS; mostly fructose-, mannose-, galactitol-, cellobiose-, and ascorbate-specific PTS systems), protein folding, and cobalamin biosynthesis were the most upregulated genes during HPP damage recovery. We observed that cell-division-related genes (divIC, dicIVA, ftsE, and ftsX) were downregulated. By contrast, peptidoglycan-synthesis genes (murG, murC, and pbp2A) were upregulated. This indicates that cell-wall repair occurs as a part of HPP damage recovery. We also observed that prophage genes, including anti-CRISPR genes, were induced by HPP. Interestingly, a large amount of RNA-seq data (up to 85%) was mapped to Rli47, which is a non-coding RNA that is upregulated after HPP. Thus, we predicted that Rli47 plays a role in HPP damage recovery in L. monocytogenes. Moreover, gene-deletion experiments showed that amongst peptidoglycan biosynthesis genes, pbp2A mutants are more sensitive to HPP. Conclusions We identified several genes and mechanisms that may play a role in recovery from HPP damage of L. monocytogenes. Our study contributes to new information on pathogen inactivation by HPP. |
| first_indexed | 2024-12-14T12:07:27Z |
| format | Article |
| id | doaj.art-f9393c656c4f4cbdbac7d25d477e56f4 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-14T12:07:27Z |
| publishDate | 2021-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-f9393c656c4f4cbdbac7d25d477e56f42022-12-21T23:01:50ZengBMCBMC Genomics1471-21642021-02-0122112010.1186/s12864-021-07407-6High-pressure processing-induced transcriptome response during recovery of Listeria monocytogenesIlhan Cem Duru0Florentina Ionela Bucur1Margarita Andreevskaya2Bahareh Nikparvar3Anne Ylinen4Leontina Grigore-Gurgu5Tone Mari Rode6Peter Crauwels7Pia Laine8Lars Paulin9Trond Løvdal10Christian U. Riedel11Nadav Bar12Daniela Borda13Anca Ioana Nicolau14Petri Auvinen15Institute of Biotechnology, University of HelsinkiFaculty of Food Science and Engineering, Dunarea de Jos University of GalatiInstitute of Biotechnology, University of HelsinkiDepartment of Chemical Engineering, Norwegian University of Science and Technology (NTNU)Institute of Biotechnology, University of HelsinkiFaculty of Food Science and Engineering, Dunarea de Jos University of GalatiDepartment of Process Technology, Nofima – Norwegian Institute of Food, Fisheries and Aquaculture ResearchInstitute of Microbiology and Biotechnology, Ulm, UniversityInstitute of Biotechnology, University of HelsinkiInstitute of Biotechnology, University of HelsinkiDepartment of Process Technology, Nofima – Norwegian Institute of Food, Fisheries and Aquaculture ResearchInstitute of Microbiology and Biotechnology, Ulm, UniversityDepartment of Chemical Engineering, Norwegian University of Science and Technology (NTNU)Faculty of Food Science and Engineering, Dunarea de Jos University of GalatiFaculty of Food Science and Engineering, Dunarea de Jos University of GalatiInstitute of Biotechnology, University of HelsinkiAbstract Background High-pressure processing (HPP) is a commonly used technique in the food industry to inactivate pathogens, including L. monocytogenes. It has been shown that L. monocytogenes is able to recover from HPP injuries and can start to grow again during long-term cold storage. To date, the gene expression profiling of L. monocytogenes during HPP damage recovery at cooling temperature has not been studied. In order identify key genes that play a role in recovery of the damage caused by HPP treatment, we performed RNA-sequencing (RNA-seq) for two L. monocytogenes strains (barotolerant RO15 and barosensitive ScottA) at nine selected time points (up to 48 h) after treatment with two pressure levels (200 and 400 MPa). Results The results showed that a general stress response was activated by SigB after HPP treatment. In addition, the phosphotransferase system (PTS; mostly fructose-, mannose-, galactitol-, cellobiose-, and ascorbate-specific PTS systems), protein folding, and cobalamin biosynthesis were the most upregulated genes during HPP damage recovery. We observed that cell-division-related genes (divIC, dicIVA, ftsE, and ftsX) were downregulated. By contrast, peptidoglycan-synthesis genes (murG, murC, and pbp2A) were upregulated. This indicates that cell-wall repair occurs as a part of HPP damage recovery. We also observed that prophage genes, including anti-CRISPR genes, were induced by HPP. Interestingly, a large amount of RNA-seq data (up to 85%) was mapped to Rli47, which is a non-coding RNA that is upregulated after HPP. Thus, we predicted that Rli47 plays a role in HPP damage recovery in L. monocytogenes. Moreover, gene-deletion experiments showed that amongst peptidoglycan biosynthesis genes, pbp2A mutants are more sensitive to HPP. Conclusions We identified several genes and mechanisms that may play a role in recovery from HPP damage of L. monocytogenes. Our study contributes to new information on pathogen inactivation by HPP.https://doi.org/10.1186/s12864-021-07407-6Time-series RNA-seqStress recoveryRli47Food pathogenSigma factor B |
| spellingShingle | Ilhan Cem Duru Florentina Ionela Bucur Margarita Andreevskaya Bahareh Nikparvar Anne Ylinen Leontina Grigore-Gurgu Tone Mari Rode Peter Crauwels Pia Laine Lars Paulin Trond Løvdal Christian U. Riedel Nadav Bar Daniela Borda Anca Ioana Nicolau Petri Auvinen High-pressure processing-induced transcriptome response during recovery of Listeria monocytogenes BMC Genomics Time-series RNA-seq Stress recovery Rli47 Food pathogen Sigma factor B |
| title | High-pressure processing-induced transcriptome response during recovery of Listeria monocytogenes |
| title_full | High-pressure processing-induced transcriptome response during recovery of Listeria monocytogenes |
| title_fullStr | High-pressure processing-induced transcriptome response during recovery of Listeria monocytogenes |
| title_full_unstemmed | High-pressure processing-induced transcriptome response during recovery of Listeria monocytogenes |
| title_short | High-pressure processing-induced transcriptome response during recovery of Listeria monocytogenes |
| title_sort | high pressure processing induced transcriptome response during recovery of listeria monocytogenes |
| topic | Time-series RNA-seq Stress recovery Rli47 Food pathogen Sigma factor B |
| url | https://doi.org/10.1186/s12864-021-07407-6 |
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