Comparison of Cas12a and Cas9-mediated mutagenesis in tomato cells
Abstract Cas12a is a promising addition to the CRISPR toolbox, offering versatility due to its TTTV-protospacer adjacent motif (PAM) and the fact that it induces double-stranded breaks (DSBs) with single-stranded overhangs. We characterized Cas12a-mediated genome editing in tomato using high-through...
| Главные авторы: | , , , , |
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| Формат: | Статья |
| Язык: | English |
| Опубликовано: |
Nature Portfolio
2024-02-01
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| Серии: | Scientific Reports |
| Online-ссылка: | https://doi.org/10.1038/s41598-024-55088-4 |