HycDemux: a hybrid unsupervised approach for accurate barcoded sample demultiplexing in nanopore sequencing

HycDemux: a hybrid unsupervised approach for accurate barcoded sample demultiplexing in nanopore sequencing

Abstract DNA barcodes enable Oxford Nanopore sequencing to sequence multiple barcoded DNA samples on a single flow cell. DNA sequences with the same barcode need to be grouped together through demultiplexing. As the number of samples increases, accurate demultiplexing becomes difficult. We introduce...

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Bibliographic Details
Main Authors: Renmin Han, Junhai Qi, Yang Xue, Xiujuan Sun, Fa Zhang, Xin Gao, Guojun Li
Format: Article
Language:English
Published: BMC 2023-10-01
Series:Genome Biology
Subjects:
Nanopore sequencing
Demultiplexing
Clustering
Online Access:https://doi.org/10.1186/s13059-023-03053-1

Internet

https://doi.org/10.1186/s13059-023-03053-1

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