Fast and efficient synaptosome isolation and post-synaptic density enrichment from hiPSC-motor neurons by biochemical sub-cellular fractionation

Fast and efficient synaptosome isolation and post-synaptic density enrichment from hiPSC-motor neurons by biochemical sub-cellular fractionation

Summary: We describe here a time-efficient, in-house protocol for synaptosome isolation and enrichment of the post-synaptic density (PSD) from hiPSC-derived motor neurons. By using biochemical sub-cellular fractionation, the crude synaptosome is first isolated from the cytosol and is then further se...

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Bibliographic Details
Main Authors: Sandeep Rajkumar, Tobias M. Böckers, Alberto Catanese
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Cell culture
Cell separation/fractionation
Neuroscience
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723000199

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723000199

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