Duration of fermentation affects microbiome composition and biological activity of an Indian traditional formulation – Panchagavya
Objective: This study aimed at investigating whether the duration of fermenting Panchagavya (PG) preparation in copper vessel affects its biological activity and microbiome composition. Materials and methods: Prophylactic potential of PG against bacterial infection was assessed through an in vivo as...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Journal of Ayurveda and Integrative Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0975947623001973 |
| _version_ | 1797267919842836480 |
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| author | Gemini Gajera Snehal Funde Hanmanthrao Palep Vijay Kothari |
| author_facet | Gemini Gajera Snehal Funde Hanmanthrao Palep Vijay Kothari |
| author_sort | Gemini Gajera |
| collection | DOAJ |
| description | Objective: This study aimed at investigating whether the duration of fermenting Panchagavya (PG) preparation in copper vessel affects its biological activity and microbiome composition. Materials and methods: Prophylactic potential of PG against bacterial infection was assessed through an in vivo assay employing the nematode worm Caenorhabditis elegans as a model host. Bacterial diversity of the PG samples was revealed through metagenomic analysis. Results: Duration of fermentation was found to affect biological activity as well as microbiome composition of the PG samples. PG-samples fermented ≥60 min lost their prophylactic potential, and develop anthelmintic activity. Bacterial phyla whose relative abundance was significantly different between the prophylactic and anthelmintic PG samples were Planctomycetota, Proteabacteria, Bacteroidota, Verrucomicrobiota, Patescibacteria, Acidobacteriota, Chloroflexi, Firmicutes and Campilobacterota. Conclusion: This study validates the prophylactic potential of Panchagavya against bacterial pathogens, and shows that duration of the fermentation time while preparing PG can have profound effect on its biological activities. Biological activities of PG samples seem to have a correlation with their inherent microbial community. Metagenomic profiling can be an effective tool for standardization of PG formulations. |
| first_indexed | 2024-04-25T01:24:14Z |
| format | Article |
| id | doaj.art-f962cab295b64b0e952569f0022d1d00 |
| institution | Directory Open Access Journal |
| issn | 0975-9476 |
| language | English |
| last_indexed | 2024-04-25T01:24:14Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ayurveda and Integrative Medicine |
| spelling | doaj.art-f962cab295b64b0e952569f0022d1d002024-03-09T09:24:11ZengElsevierJournal of Ayurveda and Integrative Medicine0975-94762024-03-01152100880Duration of fermentation affects microbiome composition and biological activity of an Indian traditional formulation – PanchagavyaGemini Gajera0Snehal Funde1Hanmanthrao Palep2Vijay Kothari3Institute of Science, Nirma University, Ahmedabad, 382481, IndiaDr. Palep's Medical Research Foundation, Mumbai, IndiaDr. Palep's Medical Research Foundation, Mumbai, IndiaInstitute of Science, Nirma University, Ahmedabad, 382481, India; Corresponding author.Objective: This study aimed at investigating whether the duration of fermenting Panchagavya (PG) preparation in copper vessel affects its biological activity and microbiome composition. Materials and methods: Prophylactic potential of PG against bacterial infection was assessed through an in vivo assay employing the nematode worm Caenorhabditis elegans as a model host. Bacterial diversity of the PG samples was revealed through metagenomic analysis. Results: Duration of fermentation was found to affect biological activity as well as microbiome composition of the PG samples. PG-samples fermented ≥60 min lost their prophylactic potential, and develop anthelmintic activity. Bacterial phyla whose relative abundance was significantly different between the prophylactic and anthelmintic PG samples were Planctomycetota, Proteabacteria, Bacteroidota, Verrucomicrobiota, Patescibacteria, Acidobacteriota, Chloroflexi, Firmicutes and Campilobacterota. Conclusion: This study validates the prophylactic potential of Panchagavya against bacterial pathogens, and shows that duration of the fermentation time while preparing PG can have profound effect on its biological activities. Biological activities of PG samples seem to have a correlation with their inherent microbial community. Metagenomic profiling can be an effective tool for standardization of PG formulations.http://www.sciencedirect.com/science/article/pii/S0975947623001973AnthelminticAntibiotic-resistant bacteriaMetagenomicsPanchagavya microbiome |
| spellingShingle | Gemini Gajera Snehal Funde Hanmanthrao Palep Vijay Kothari Duration of fermentation affects microbiome composition and biological activity of an Indian traditional formulation – Panchagavya Journal of Ayurveda and Integrative Medicine Anthelmintic Antibiotic-resistant bacteria Metagenomics Panchagavya microbiome |
| title | Duration of fermentation affects microbiome composition and biological activity of an Indian traditional formulation – Panchagavya |
| title_full | Duration of fermentation affects microbiome composition and biological activity of an Indian traditional formulation – Panchagavya |
| title_fullStr | Duration of fermentation affects microbiome composition and biological activity of an Indian traditional formulation – Panchagavya |
| title_full_unstemmed | Duration of fermentation affects microbiome composition and biological activity of an Indian traditional formulation – Panchagavya |
| title_short | Duration of fermentation affects microbiome composition and biological activity of an Indian traditional formulation – Panchagavya |
| title_sort | duration of fermentation affects microbiome composition and biological activity of an indian traditional formulation panchagavya |
| topic | Anthelmintic Antibiotic-resistant bacteria Metagenomics Panchagavya microbiome |
| url | http://www.sciencedirect.com/science/article/pii/S0975947623001973 |
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