The association between osteopontin gene polymorphisms, osteopontin expression and sarcoidosis.
BACKGROUND:Sarcoidosis is a systemic inflammatory disease of unknown etiology. Osteopontin (SPP1, OPN) is an extra cellular matrix glycoprotein and cytokine with a known role in granuloma formation and in autoimmune and inflammatory diseases. OBJECTIVE:To determine whether plasma OPN levels are elev...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5333822?pdf=render |
| _version_ | 1811199791515828224 |
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| author | Hadas Lavi Miri Assayag Assaf Schwartz Nissim Arish Zvi G Fridlender Neville Berkman |
| author_facet | Hadas Lavi Miri Assayag Assaf Schwartz Nissim Arish Zvi G Fridlender Neville Berkman |
| author_sort | Hadas Lavi |
| collection | DOAJ |
| description | BACKGROUND:Sarcoidosis is a systemic inflammatory disease of unknown etiology. Osteopontin (SPP1, OPN) is an extra cellular matrix glycoprotein and cytokine with a known role in granuloma formation and in autoimmune and inflammatory diseases. OBJECTIVE:To determine whether plasma OPN levels are elevated in patients with sarcoidosis and compare the frequency of four single nucleotide polymorphism (SNPs) variants in the OPN gene in sarcoidosis patients compared to healthy controls. METHODS:Demographic and clinical information, radiological studies and pulmonary function tests were evaluated in 113 patients with sarcoidosis and in 79 healthy controls. Blood samples were analyzed for SNPs of the OPN gene and for plasma OPN and CRP levels. Association between clinical features of disease and OPN levels as well as SNP frequencies was determined. RESULTS:Plasma OPN levels were higher in sarcoidosis patients than in healthy subjects, (median: 217 vs 122ng/ml, p<0.001). Area under the curve for receiver operator curves (ROC) was 0.798 (0.686-0.909 95% CI.) No differences were observed between sarcoidosis patients and controls in the frequency of any of the SNPs evaluated. Presence of lung parenchymal involvement was associated with SNP distribution at rs1126772 (p = 0.02). We found no correlation between SNPs distribution and plasma OPN levels. CONCLUSIONS:Osteopontin protein levels are elevated in sarcoidosis. We found no evidence for an association between SNPs on the osteopontin gene and plasma OPN levels or the presence of sarcoidosis, however, an association between genotype and several phenotypic clinical parameters of disease was observed. |
| first_indexed | 2024-04-12T01:53:16Z |
| format | Article |
| id | doaj.art-f98d08e2fe094414940efa656de57a85 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T01:53:16Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f98d08e2fe094414940efa656de57a852022-12-22T03:52:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01123e017194510.1371/journal.pone.0171945The association between osteopontin gene polymorphisms, osteopontin expression and sarcoidosis.Hadas LaviMiri AssayagAssaf SchwartzNissim ArishZvi G FridlenderNeville BerkmanBACKGROUND:Sarcoidosis is a systemic inflammatory disease of unknown etiology. Osteopontin (SPP1, OPN) is an extra cellular matrix glycoprotein and cytokine with a known role in granuloma formation and in autoimmune and inflammatory diseases. OBJECTIVE:To determine whether plasma OPN levels are elevated in patients with sarcoidosis and compare the frequency of four single nucleotide polymorphism (SNPs) variants in the OPN gene in sarcoidosis patients compared to healthy controls. METHODS:Demographic and clinical information, radiological studies and pulmonary function tests were evaluated in 113 patients with sarcoidosis and in 79 healthy controls. Blood samples were analyzed for SNPs of the OPN gene and for plasma OPN and CRP levels. Association between clinical features of disease and OPN levels as well as SNP frequencies was determined. RESULTS:Plasma OPN levels were higher in sarcoidosis patients than in healthy subjects, (median: 217 vs 122ng/ml, p<0.001). Area under the curve for receiver operator curves (ROC) was 0.798 (0.686-0.909 95% CI.) No differences were observed between sarcoidosis patients and controls in the frequency of any of the SNPs evaluated. Presence of lung parenchymal involvement was associated with SNP distribution at rs1126772 (p = 0.02). We found no correlation between SNPs distribution and plasma OPN levels. CONCLUSIONS:Osteopontin protein levels are elevated in sarcoidosis. We found no evidence for an association between SNPs on the osteopontin gene and plasma OPN levels or the presence of sarcoidosis, however, an association between genotype and several phenotypic clinical parameters of disease was observed.http://europepmc.org/articles/PMC5333822?pdf=render |
| spellingShingle | Hadas Lavi Miri Assayag Assaf Schwartz Nissim Arish Zvi G Fridlender Neville Berkman The association between osteopontin gene polymorphisms, osteopontin expression and sarcoidosis. PLoS ONE |
| title | The association between osteopontin gene polymorphisms, osteopontin expression and sarcoidosis. |
| title_full | The association between osteopontin gene polymorphisms, osteopontin expression and sarcoidosis. |
| title_fullStr | The association between osteopontin gene polymorphisms, osteopontin expression and sarcoidosis. |
| title_full_unstemmed | The association between osteopontin gene polymorphisms, osteopontin expression and sarcoidosis. |
| title_short | The association between osteopontin gene polymorphisms, osteopontin expression and sarcoidosis. |
| title_sort | association between osteopontin gene polymorphisms osteopontin expression and sarcoidosis |
| url | http://europepmc.org/articles/PMC5333822?pdf=render |
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