A Ponceau S Staining-Based Dot Blot Assay for Rapid Protein Quantification of Biological Samples

Despite the availability of a wide range of commercial kits, protein quantification is often unreliable, especially for tissue-derived samples, leading to uneven loading in subsequent experiments. Here we show that the widely used Bicinchoninic Acid (BCA) assay tends to underestimate protein concent...

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Bibliographic Details
Main Authors: Dario Lucas Helbing, Leopold Böhm, Nova Oraha, Leonie Karoline Stabenow, Yan Cui
Format: Article
Language:English
Published: MDPI AG 2022-01-01
Series:Gels
Subjects:
Online Access:https://www.mdpi.com/2310-2861/8/1/43