Particle-based phasor-FLIM-FRET resolves protein-protein interactions inside single viral particles

Fluorescence lifetime imaging microscopy (FLIM) is a popular modality to create additional contrast in fluorescence images. By carefully analyzing pixel-based nanosecond lifetime patterns, FLIM allows studying complex molecular populations. At the single-molecule or single-particle level, however, i...

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Bibliographic Details
Main Authors: Quinten Coucke, Nagma Parveen, Guillermo Solís Fernández, Chen Qian, Johan Hofkens, Zeger Debyser, Jelle Hendrix
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:Biophysical Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S266707472300023X