CD115+ monocytes protect microbially experienced mice against E. coli-induced sepsis
Summary: Uropathogenic E. coli (UPEC) is a primary organism responsible for urinary tract infections and a common cause of sepsis. Microbially experienced laboratory mice, generated by cohousing with pet store mice, exhibit increased morbidity and mortality to polymicrobial sepsis or lipopolysacchar...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723013578 |
| _version_ | 1827630411284480000 |
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| author | Matthew D. Martin Cara Skon-Hegg Caleb Y. Kim Julie Xu Tamara A. Kucaba Whitney Swanson Mark J. Pierson Jesse W. Williams Vladimir P. Badovinac Steven S. Shen Molly A. Ingersoll Thomas S. Griffith |
| author_facet | Matthew D. Martin Cara Skon-Hegg Caleb Y. Kim Julie Xu Tamara A. Kucaba Whitney Swanson Mark J. Pierson Jesse W. Williams Vladimir P. Badovinac Steven S. Shen Molly A. Ingersoll Thomas S. Griffith |
| author_sort | Matthew D. Martin |
| collection | DOAJ |
| description | Summary: Uropathogenic E. coli (UPEC) is a primary organism responsible for urinary tract infections and a common cause of sepsis. Microbially experienced laboratory mice, generated by cohousing with pet store mice, exhibit increased morbidity and mortality to polymicrobial sepsis or lipopolysaccharide challenge. By contrast, cohoused mice display significant resistance, compared with specific pathogen-free mice, to a monomicrobial sepsis model using UPEC. CD115+ monocytes mediate protection in the cohoused mice, as depletion of these cells leads to increased mortality and UPEC pathogen burden. Further study of the cohoused mice reveals increased TNF-α production by monocytes, a skewing toward Ly6ChiCD115+ “classical” monocytes, and enhanced egress of Ly6ChiCD115+ monocytes from the bone marrow. Analysis of cohoused bone marrow also finds increased frequency and number of myeloid multipotent progenitor cells. These results show that a history of microbial exposure impacts innate immunity in mice, which can have important implications for the preclinical study of sepsis. |
| first_indexed | 2024-03-09T14:05:23Z |
| format | Article |
| id | doaj.art-fa229195bef94cf2b86c1db7a617d692 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-09T14:05:23Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-fa229195bef94cf2b86c1db7a617d6922023-11-30T05:06:52ZengElsevierCell Reports2211-12472023-11-014211113345CD115+ monocytes protect microbially experienced mice against E. coli-induced sepsisMatthew D. Martin0Cara Skon-Hegg1Caleb Y. Kim2Julie Xu3Tamara A. Kucaba4Whitney Swanson5Mark J. Pierson6Jesse W. Williams7Vladimir P. Badovinac8Steven S. Shen9Molly A. Ingersoll10Thomas S. Griffith11Department of Urology, University of Minnesota, Minneapolis, MN 55455, USA; Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USADepartment of Urology, University of Minnesota, Minneapolis, MN 55455, USA; Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USACenter for Immunology, University of Minnesota, Minneapolis, MN 55455, USA; Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455, USADepartment of Urology, University of Minnesota, Minneapolis, MN 55455, USADepartment of Urology, University of Minnesota, Minneapolis, MN 55455, USADepartment of Urology, University of Minnesota, Minneapolis, MN 55455, USACenter for Immunology, University of Minnesota, Minneapolis, MN 55455, USA; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455, USACenter for Immunology, University of Minnesota, Minneapolis, MN 55455, USA; Department of Integrative Biology and Physiology, University of Minnesota, Minneapolis, MN 55455, USADepartment of Pathology, University of Iowa, Iowa City, IA 52242, USA; Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA 52242, USAInstitute for Health Informatics, University of Minnesota, Minneapolis, MN 55455, USAUniversité Paris Cité, Institut Cochin, INSERM U1016, CNRS UMR 8104, 75014 Paris, France; Mucosal Inflammation and Immunity, Department of Immunology, Institut Pasteur, Inserm U1223, 75015 Paris, FranceDepartment of Urology, University of Minnesota, Minneapolis, MN 55455, USA; Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USA; Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455, USA; Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA; Minneapolis VA Health Care System, Minneapolis, MN 55417, USA; Corresponding authorSummary: Uropathogenic E. coli (UPEC) is a primary organism responsible for urinary tract infections and a common cause of sepsis. Microbially experienced laboratory mice, generated by cohousing with pet store mice, exhibit increased morbidity and mortality to polymicrobial sepsis or lipopolysaccharide challenge. By contrast, cohoused mice display significant resistance, compared with specific pathogen-free mice, to a monomicrobial sepsis model using UPEC. CD115+ monocytes mediate protection in the cohoused mice, as depletion of these cells leads to increased mortality and UPEC pathogen burden. Further study of the cohoused mice reveals increased TNF-α production by monocytes, a skewing toward Ly6ChiCD115+ “classical” monocytes, and enhanced egress of Ly6ChiCD115+ monocytes from the bone marrow. Analysis of cohoused bone marrow also finds increased frequency and number of myeloid multipotent progenitor cells. These results show that a history of microbial exposure impacts innate immunity in mice, which can have important implications for the preclinical study of sepsis.http://www.sciencedirect.com/science/article/pii/S2211124723013578CP: MicrobiologyCP: Immunology |
| spellingShingle | Matthew D. Martin Cara Skon-Hegg Caleb Y. Kim Julie Xu Tamara A. Kucaba Whitney Swanson Mark J. Pierson Jesse W. Williams Vladimir P. Badovinac Steven S. Shen Molly A. Ingersoll Thomas S. Griffith CD115+ monocytes protect microbially experienced mice against E. coli-induced sepsis Cell Reports CP: Microbiology CP: Immunology |
| title | CD115+ monocytes protect microbially experienced mice against E. coli-induced sepsis |
| title_full | CD115+ monocytes protect microbially experienced mice against E. coli-induced sepsis |
| title_fullStr | CD115+ monocytes protect microbially experienced mice against E. coli-induced sepsis |
| title_full_unstemmed | CD115+ monocytes protect microbially experienced mice against E. coli-induced sepsis |
| title_short | CD115+ monocytes protect microbially experienced mice against E. coli-induced sepsis |
| title_sort | cd115 monocytes protect microbially experienced mice against e coli induced sepsis |
| topic | CP: Microbiology CP: Immunology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723013578 |
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