Detection of Quantitative Trait Loci Controlling the Content of Phenolic Compounds in an Asian Plum (Prunus salicina L.) F1 Population
Consumption of fresh fruit is known to protect against non-communicable diseases due to the fruit's content in compounds with an antioxidant capacity, among them is polyphenols. Asian plums (Prunus salicina L.) accumulate more than 40 phenolic compounds, with a remarkable diversity in their pro...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.679059/full |
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| author | Diego Valderrama-Soto Juan Salazar Ailynne Sepúlveda-González Claudia Silva-Andrade Claudio Gardana Héctor Morales Benjamin Battistoni Benjamin Battistoni Pablo Jiménez-Muñoz Pablo Jiménez-Muñoz Mauricio González Mauricio González Álvaro Peña-Neira Rodrigo Infante Igor Pacheco |
| author_facet | Diego Valderrama-Soto Juan Salazar Ailynne Sepúlveda-González Claudia Silva-Andrade Claudio Gardana Héctor Morales Benjamin Battistoni Benjamin Battistoni Pablo Jiménez-Muñoz Pablo Jiménez-Muñoz Mauricio González Mauricio González Álvaro Peña-Neira Rodrigo Infante Igor Pacheco |
| author_sort | Diego Valderrama-Soto |
| collection | DOAJ |
| description | Consumption of fresh fruit is known to protect against non-communicable diseases due to the fruit's content in compounds with an antioxidant capacity, among them is polyphenols. Asian plums (Prunus salicina L.) accumulate more than 40 phenolic compounds, with a remarkable diversity in their profiles, depending on the variety and environmental conditions. Although candidate genes have been indicated to control this trait, the loci controlling its phenotypic variation have not yet been defined in this species. The aim of this work was to identify the quantitative trait Loci (QTL) controlling the phenolic compounds content in the Asian plum skin and flesh. Using UHPLC-DAD-Orbitrap-MS, we determined that cyanidin-3-glucoside and cyanidin-3-rutinoside are the main anthocyanins in Asian plums. Other anthocyanins found to a lesser extent were tentatively identified as cyanidin bound to different sugar and procyanidin moieties. Then we phenotyped fruits of 92 and 80 F1 seedlings from the cross < “98.99” × “Angeleno”> (98 Ang) for two harvest seasons. We used HPLC-DAD to quantify single anthocyanin and spectrophotometric techniques to determine the total content of phenols, flavonoids, procyanidins, and antioxidant activity (DPPH and FRAP). To determine the phenotype-genotype association of phenolic compounds content, phenotypic values (adjusted by linear mixed-effects models), genotypic data and linkage maps were analyzed with the multiple QTL model (MQM) approach. We found a total of 21 significant trait-marker associations: 13 QTLs segregating from “98.99” and 8 QTLs from “Angeleno.” From these associations, 8 corresponded to phenolic compound content in the flesh and 13 in the skin. Phenotype variance was explained by the detected loci, ranging from 12.4 to 27.1%. The identified loci are related to the content of cyanidin-3-glucoside (LG4), cyanidin-3-rutinoside (LG4), total flavonoids and procyanidins (LG5 and LG8), and minor anthocyanin compounds (LG3 and LG4). These results will help improve the efficiency of breeding programs for the generation of Asian plum varieties with high phenolic compound content. |
| first_indexed | 2024-12-17T06:50:22Z |
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| language | English |
| last_indexed | 2024-12-17T06:50:22Z |
| publishDate | 2021-07-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-fa3d9d0ccee2420488f1fc4bbae5b83f2022-12-21T21:59:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-07-011210.3389/fpls.2021.679059679059Detection of Quantitative Trait Loci Controlling the Content of Phenolic Compounds in an Asian Plum (Prunus salicina L.) F1 PopulationDiego Valderrama-Soto0Juan Salazar1Ailynne Sepúlveda-González2Claudia Silva-Andrade3Claudio Gardana4Héctor Morales5Benjamin Battistoni6Benjamin Battistoni7Pablo Jiménez-Muñoz8Pablo Jiménez-Muñoz9Mauricio González10Mauricio González11Álvaro Peña-Neira12Rodrigo Infante13Igor Pacheco14Instituto de Nutrición y Tecnología de Alimentos (INTA), Universidad de Chile, Santiago, ChileDepartment of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas (CEBAS-CSIC), Murcia, SpainInstituto de Nutrición y Tecnología de Alimentos (INTA), Universidad de Chile, Santiago, ChileLaboratorio de Biología de Redes, Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Santiago, ChileDipartimento di Scienze per gli Alimenti, la Nutrizione, l'Ambiente, Università degli Studi di Milano, Milan, ItalyDepartamento de Agroindustria y Enología, Universidad de Chile, Santiago, ChileInstituto de Nutrición y Tecnología de Alimentos (INTA), Universidad de Chile, Santiago, ChilePrograma de Doctorado en Ciencias Silvoagropecuarias y Veterinarias, Campus Sur Universidad de Chile, Santiago, ChileInstituto de Nutrición y Tecnología de Alimentos (INTA), Universidad de Chile, Santiago, ChileDepartamento de Producción Agrícola, Universidad de Chile, Santiago, ChileInstituto de Nutrición y Tecnología de Alimentos (INTA), Universidad de Chile, Santiago, ChileCenter for Genome Regulation (CGR), Santiago, ChileDepartamento de Agroindustria y Enología, Universidad de Chile, Santiago, ChileDepartamento de Producción Agrícola, Universidad de Chile, Santiago, ChileInstituto de Nutrición y Tecnología de Alimentos (INTA), Universidad de Chile, Santiago, ChileConsumption of fresh fruit is known to protect against non-communicable diseases due to the fruit's content in compounds with an antioxidant capacity, among them is polyphenols. Asian plums (Prunus salicina L.) accumulate more than 40 phenolic compounds, with a remarkable diversity in their profiles, depending on the variety and environmental conditions. Although candidate genes have been indicated to control this trait, the loci controlling its phenotypic variation have not yet been defined in this species. The aim of this work was to identify the quantitative trait Loci (QTL) controlling the phenolic compounds content in the Asian plum skin and flesh. Using UHPLC-DAD-Orbitrap-MS, we determined that cyanidin-3-glucoside and cyanidin-3-rutinoside are the main anthocyanins in Asian plums. Other anthocyanins found to a lesser extent were tentatively identified as cyanidin bound to different sugar and procyanidin moieties. Then we phenotyped fruits of 92 and 80 F1 seedlings from the cross < “98.99” × “Angeleno”> (98 Ang) for two harvest seasons. We used HPLC-DAD to quantify single anthocyanin and spectrophotometric techniques to determine the total content of phenols, flavonoids, procyanidins, and antioxidant activity (DPPH and FRAP). To determine the phenotype-genotype association of phenolic compounds content, phenotypic values (adjusted by linear mixed-effects models), genotypic data and linkage maps were analyzed with the multiple QTL model (MQM) approach. We found a total of 21 significant trait-marker associations: 13 QTLs segregating from “98.99” and 8 QTLs from “Angeleno.” From these associations, 8 corresponded to phenolic compound content in the flesh and 13 in the skin. Phenotype variance was explained by the detected loci, ranging from 12.4 to 27.1%. The identified loci are related to the content of cyanidin-3-glucoside (LG4), cyanidin-3-rutinoside (LG4), total flavonoids and procyanidins (LG5 and LG8), and minor anthocyanin compounds (LG3 and LG4). These results will help improve the efficiency of breeding programs for the generation of Asian plum varieties with high phenolic compound content.https://www.frontiersin.org/articles/10.3389/fpls.2021.679059/fullQuantitative Trait Lociphenolic compoundsPrunus salicinaantioxidant activityflavonoidsprocyanidins |
| spellingShingle | Diego Valderrama-Soto Juan Salazar Ailynne Sepúlveda-González Claudia Silva-Andrade Claudio Gardana Héctor Morales Benjamin Battistoni Benjamin Battistoni Pablo Jiménez-Muñoz Pablo Jiménez-Muñoz Mauricio González Mauricio González Álvaro Peña-Neira Rodrigo Infante Igor Pacheco Detection of Quantitative Trait Loci Controlling the Content of Phenolic Compounds in an Asian Plum (Prunus salicina L.) F1 Population Frontiers in Plant Science Quantitative Trait Loci phenolic compounds Prunus salicina antioxidant activity flavonoids procyanidins |
| title | Detection of Quantitative Trait Loci Controlling the Content of Phenolic Compounds in an Asian Plum (Prunus salicina L.) F1 Population |
| title_full | Detection of Quantitative Trait Loci Controlling the Content of Phenolic Compounds in an Asian Plum (Prunus salicina L.) F1 Population |
| title_fullStr | Detection of Quantitative Trait Loci Controlling the Content of Phenolic Compounds in an Asian Plum (Prunus salicina L.) F1 Population |
| title_full_unstemmed | Detection of Quantitative Trait Loci Controlling the Content of Phenolic Compounds in an Asian Plum (Prunus salicina L.) F1 Population |
| title_short | Detection of Quantitative Trait Loci Controlling the Content of Phenolic Compounds in an Asian Plum (Prunus salicina L.) F1 Population |
| title_sort | detection of quantitative trait loci controlling the content of phenolic compounds in an asian plum prunus salicina l f1 population |
| topic | Quantitative Trait Loci phenolic compounds Prunus salicina antioxidant activity flavonoids procyanidins |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.679059/full |
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