Development of milk powder containing Lactobacillus plantarum NCIMB 8826 immobilized with prebiotic hi-maize starch and survival under simulated gastric and intestinal conditions
Abstract The objectives of this study were to develop a probiotic milk powder containing Lactobacillus plantarum NCIMB 8826 immobilized with prebiotic Hi-maize starch and to analyze cell viability after spray drying and exposure to simulated gastric and intestinal conditions. Milk powders containing...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-11-01
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| Series: | Food Production, Processing and Nutrition |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s43014-019-0011-6 |
| _version_ | 1818019804903112704 |
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| author | Ronamae Bradford Vondel Reyes Franklin Bonilla Federico Bueno Bennett Dzandu Chen Liu Alexander Chouljenko Subramaniam Sathivel |
| author_facet | Ronamae Bradford Vondel Reyes Franklin Bonilla Federico Bueno Bennett Dzandu Chen Liu Alexander Chouljenko Subramaniam Sathivel |
| author_sort | Ronamae Bradford |
| collection | DOAJ |
| description | Abstract The objectives of this study were to develop a probiotic milk powder containing Lactobacillus plantarum NCIMB 8826 immobilized with prebiotic Hi-maize starch and to analyze cell viability after spray drying and exposure to simulated gastric and intestinal conditions. Milk powders containing free L. plantarum and cells immobilized with Hi-maize starch were assessed. Powders were evaluated during storage at 4 °C for 15 days. After spray drying, at 0 and 15 days of storage both treatments had over 8 log CFU/g of viable cells and there were higher viable counts found for immobilized cells compared to free cells after 120 min in simulated gastric fluid. At 15 days of storage, immobilized cells had higher viable counts than free cells after exposure to simulated intestinal fluid for 120 min. The combined probiotic and prebiotic milk powder had stable viable cell counts at refrigerated storage conditions and under simulated gastric and intestinal transit. Graphical abstract |
| first_indexed | 2024-04-14T07:57:11Z |
| format | Article |
| id | doaj.art-fa4eb126893f4ba19286c6f3d808dc90 |
| institution | Directory Open Access Journal |
| issn | 2661-8974 |
| language | English |
| last_indexed | 2024-04-14T07:57:11Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Food Production, Processing and Nutrition |
| spelling | doaj.art-fa4eb126893f4ba19286c6f3d808dc902022-12-22T02:05:01ZengBMCFood Production, Processing and Nutrition2661-89742019-11-01111810.1186/s43014-019-0011-6Development of milk powder containing Lactobacillus plantarum NCIMB 8826 immobilized with prebiotic hi-maize starch and survival under simulated gastric and intestinal conditionsRonamae Bradford0Vondel Reyes1Franklin Bonilla2Federico Bueno3Bennett Dzandu4Chen Liu5Alexander Chouljenko6Subramaniam Sathivel7Department of Chemistry, University of the West IndiesSchool of Nutrition and Food Sciences, 220 H.D. Wilson Laboratories, Louisiana State University Agricultural CenterSchool of Nutrition and Food Sciences, 220 H.D. Wilson Laboratories, Louisiana State University Agricultural CenterSchool of Nutrition and Food Sciences, 220 H.D. Wilson Laboratories, Louisiana State University Agricultural CenterSchool of Nutrition and Food Sciences, 220 H.D. Wilson Laboratories, Louisiana State University Agricultural CenterSchool of Nutrition and Food Sciences, 220 H.D. Wilson Laboratories, Louisiana State University Agricultural CenterSchool of Nutrition and Food Sciences, 220 H.D. Wilson Laboratories, Louisiana State University Agricultural CenterSchool of Nutrition and Food Sciences, 220 H.D. Wilson Laboratories, Louisiana State University Agricultural CenterAbstract The objectives of this study were to develop a probiotic milk powder containing Lactobacillus plantarum NCIMB 8826 immobilized with prebiotic Hi-maize starch and to analyze cell viability after spray drying and exposure to simulated gastric and intestinal conditions. Milk powders containing free L. plantarum and cells immobilized with Hi-maize starch were assessed. Powders were evaluated during storage at 4 °C for 15 days. After spray drying, at 0 and 15 days of storage both treatments had over 8 log CFU/g of viable cells and there were higher viable counts found for immobilized cells compared to free cells after 120 min in simulated gastric fluid. At 15 days of storage, immobilized cells had higher viable counts than free cells after exposure to simulated intestinal fluid for 120 min. The combined probiotic and prebiotic milk powder had stable viable cell counts at refrigerated storage conditions and under simulated gastric and intestinal transit. Graphical abstracthttp://link.springer.com/article/10.1186/s43014-019-0011-6ProbioticsPrebioticsSimulated gastrointestinal conditionsMilk powderSpray dryingImmobilization |
| spellingShingle | Ronamae Bradford Vondel Reyes Franklin Bonilla Federico Bueno Bennett Dzandu Chen Liu Alexander Chouljenko Subramaniam Sathivel Development of milk powder containing Lactobacillus plantarum NCIMB 8826 immobilized with prebiotic hi-maize starch and survival under simulated gastric and intestinal conditions Food Production, Processing and Nutrition Probiotics Prebiotics Simulated gastrointestinal conditions Milk powder Spray drying Immobilization |
| title | Development of milk powder containing Lactobacillus plantarum NCIMB 8826 immobilized with prebiotic hi-maize starch and survival under simulated gastric and intestinal conditions |
| title_full | Development of milk powder containing Lactobacillus plantarum NCIMB 8826 immobilized with prebiotic hi-maize starch and survival under simulated gastric and intestinal conditions |
| title_fullStr | Development of milk powder containing Lactobacillus plantarum NCIMB 8826 immobilized with prebiotic hi-maize starch and survival under simulated gastric and intestinal conditions |
| title_full_unstemmed | Development of milk powder containing Lactobacillus plantarum NCIMB 8826 immobilized with prebiotic hi-maize starch and survival under simulated gastric and intestinal conditions |
| title_short | Development of milk powder containing Lactobacillus plantarum NCIMB 8826 immobilized with prebiotic hi-maize starch and survival under simulated gastric and intestinal conditions |
| title_sort | development of milk powder containing lactobacillus plantarum ncimb 8826 immobilized with prebiotic hi maize starch and survival under simulated gastric and intestinal conditions |
| topic | Probiotics Prebiotics Simulated gastrointestinal conditions Milk powder Spray drying Immobilization |
| url | http://link.springer.com/article/10.1186/s43014-019-0011-6 |
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