Stage-Dependent Increase of Systemic Immune Activation and CCR5<sup>+</sup>CD4<sup>+</sup> T Cells in Filarial Driven Lymphedema in Ghana and Tanzania
Chronic lymphedema caused by infection of <i>Wuchereria bancrofti</i> is a disfiguring disease that leads to physical disability, stigmatization, and reduced quality of life. The edematous changes occur mainly on the lower extremities and can progress over time due to secondary bacterial...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/6/809 |
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| author | Abu Abudu Rahamani Sacha Horn Manuel Ritter Anja Feichtner Jubin Osei-Mensah Vera Serwaa Opoku Linda Batsa Debrah Thomas F. Marandu Antelmo Haule Jacklina Mhidze Abdallah Ngenya Max Demetrius Ute Klarmann-Schulz Michael Hoelscher Christof Geldmacher Achim Hoerauf Akili Kalinga Alexander Y. Debrah Inge Kroidl |
| author_facet | Abu Abudu Rahamani Sacha Horn Manuel Ritter Anja Feichtner Jubin Osei-Mensah Vera Serwaa Opoku Linda Batsa Debrah Thomas F. Marandu Antelmo Haule Jacklina Mhidze Abdallah Ngenya Max Demetrius Ute Klarmann-Schulz Michael Hoelscher Christof Geldmacher Achim Hoerauf Akili Kalinga Alexander Y. Debrah Inge Kroidl |
| author_sort | Abu Abudu Rahamani |
| collection | DOAJ |
| description | Chronic lymphedema caused by infection of <i>Wuchereria bancrofti</i> is a disfiguring disease that leads to physical disability, stigmatization, and reduced quality of life. The edematous changes occur mainly on the lower extremities and can progress over time due to secondary bacterial infections. In this study, we characterized participants with filarial lymphedema from Ghana and Tanzania as having low (stage 1–2), intermediate (stage 3–4), or advanced (stage 5–7) lymphedema to determine CD4<sup>+</sup> T cell activation patterns and markers associated with immune cell exhaustion. A flow cytometry-based analysis of peripheral whole blood revealed different T cell phenotypes within participants with different stages of filarial lymphedema. In detail, increased frequencies of CD4<sup>+</sup>HLA-DR<sup>+</sup>CD38<sup>+</sup> T cells were associated with higher stages of filarial lymphedema in patients from Ghana and Tanzania. In addition, significantly increased frequencies of CCR5<sup>+</sup>CD4<sup>+</sup> T cells were seen in Ghanaian participants with advanced LE stages, which was not observed in the Tanzanian cohort. The frequencies of CD8<sup>+</sup>PD-1<sup>+</sup> T cells were augmented in individuals with higher stage lymphedema in both countries. These findings show distinct activation and exhaustion patterns in lymphedema patients but reveal that immunological findings differ between West and East African countries. |
| first_indexed | 2024-03-11T02:04:51Z |
| format | Article |
| id | doaj.art-fa52625b0ed142b8815d82a630f8ac5e |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-11T02:04:51Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-fa52625b0ed142b8815d82a630f8ac5e2023-11-18T12:00:14ZengMDPI AGPathogens2076-08172023-06-0112680910.3390/pathogens12060809Stage-Dependent Increase of Systemic Immune Activation and CCR5<sup>+</sup>CD4<sup>+</sup> T Cells in Filarial Driven Lymphedema in Ghana and TanzaniaAbu Abudu Rahamani0Sacha Horn1Manuel Ritter2Anja Feichtner3Jubin Osei-Mensah4Vera Serwaa Opoku5Linda Batsa Debrah6Thomas F. Marandu7Antelmo Haule8Jacklina Mhidze9Abdallah Ngenya10Max Demetrius11Ute Klarmann-Schulz12Michael Hoelscher13Christof Geldmacher14Achim Hoerauf15Akili Kalinga16Alexander Y. Debrah17Inge Kroidl18Filariasis Unit, Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), UPO, PMB, Kumasi 00233, GhanaDivision of Infectious Diseases and Tropical Medicine, University Hospital Munich, Ludwig-Maximilians-Universität (LMU), 80802 Munich, GermanyInstitute for Medical Microbiology, Immunology and Parasitology (IMMIP), University Hospital Bonn, 53127 Bonn, GermanyDivision of Infectious Diseases and Tropical Medicine, University Hospital Munich, Ludwig-Maximilians-Universität (LMU), 80802 Munich, GermanyFilariasis Unit, Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), UPO, PMB, Kumasi 00233, GhanaFilariasis Unit, Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), UPO, PMB, Kumasi 00233, GhanaFilariasis Unit, Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), UPO, PMB, Kumasi 00233, GhanaMbeya Medical Research Center (MMRC), Department of Immunology, National Institute for Medical Research (NIMR), Mbeya 53107, TanzaniaMbeya Medical Research Center (MMRC), Department of Immunology, National Institute for Medical Research (NIMR), Mbeya 53107, TanzaniaMbeya Medical Research Center (MMRC), Department of Immunology, National Institute for Medical Research (NIMR), Mbeya 53107, TanzaniaNational Institute of Medical Research (NIMR)—Headquarters, Dar es Salaam 11101, TanzaniaNational Institute of Medical Research (NIMR)—Headquarters, Dar es Salaam 11101, TanzaniaInstitute for Medical Microbiology, Immunology and Parasitology (IMMIP), University Hospital Bonn, 53127 Bonn, GermanyDivision of Infectious Diseases and Tropical Medicine, University Hospital Munich, Ludwig-Maximilians-Universität (LMU), 80802 Munich, GermanyDivision of Infectious Diseases and Tropical Medicine, University Hospital Munich, Ludwig-Maximilians-Universität (LMU), 80802 Munich, GermanyInstitute for Medical Microbiology, Immunology and Parasitology (IMMIP), University Hospital Bonn, 53127 Bonn, GermanyNational Institute of Medical Research (NIMR)—Headquarters, Dar es Salaam 11101, TanzaniaFilariasis Unit, Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), UPO, PMB, Kumasi 00233, GhanaDivision of Infectious Diseases and Tropical Medicine, University Hospital Munich, Ludwig-Maximilians-Universität (LMU), 80802 Munich, GermanyChronic lymphedema caused by infection of <i>Wuchereria bancrofti</i> is a disfiguring disease that leads to physical disability, stigmatization, and reduced quality of life. The edematous changes occur mainly on the lower extremities and can progress over time due to secondary bacterial infections. In this study, we characterized participants with filarial lymphedema from Ghana and Tanzania as having low (stage 1–2), intermediate (stage 3–4), or advanced (stage 5–7) lymphedema to determine CD4<sup>+</sup> T cell activation patterns and markers associated with immune cell exhaustion. A flow cytometry-based analysis of peripheral whole blood revealed different T cell phenotypes within participants with different stages of filarial lymphedema. In detail, increased frequencies of CD4<sup>+</sup>HLA-DR<sup>+</sup>CD38<sup>+</sup> T cells were associated with higher stages of filarial lymphedema in patients from Ghana and Tanzania. In addition, significantly increased frequencies of CCR5<sup>+</sup>CD4<sup>+</sup> T cells were seen in Ghanaian participants with advanced LE stages, which was not observed in the Tanzanian cohort. The frequencies of CD8<sup>+</sup>PD-1<sup>+</sup> T cells were augmented in individuals with higher stage lymphedema in both countries. These findings show distinct activation and exhaustion patterns in lymphedema patients but reveal that immunological findings differ between West and East African countries.https://www.mdpi.com/2076-0817/12/6/809CD4<sup>+</sup> T cell activationlymphatic filariasislymphedemaCCR5PD-1HLADR/CD38 |
| spellingShingle | Abu Abudu Rahamani Sacha Horn Manuel Ritter Anja Feichtner Jubin Osei-Mensah Vera Serwaa Opoku Linda Batsa Debrah Thomas F. Marandu Antelmo Haule Jacklina Mhidze Abdallah Ngenya Max Demetrius Ute Klarmann-Schulz Michael Hoelscher Christof Geldmacher Achim Hoerauf Akili Kalinga Alexander Y. Debrah Inge Kroidl Stage-Dependent Increase of Systemic Immune Activation and CCR5<sup>+</sup>CD4<sup>+</sup> T Cells in Filarial Driven Lymphedema in Ghana and Tanzania Pathogens CD4<sup>+</sup> T cell activation lymphatic filariasis lymphedema CCR5 PD-1 HLADR/CD38 |
| title | Stage-Dependent Increase of Systemic Immune Activation and CCR5<sup>+</sup>CD4<sup>+</sup> T Cells in Filarial Driven Lymphedema in Ghana and Tanzania |
| title_full | Stage-Dependent Increase of Systemic Immune Activation and CCR5<sup>+</sup>CD4<sup>+</sup> T Cells in Filarial Driven Lymphedema in Ghana and Tanzania |
| title_fullStr | Stage-Dependent Increase of Systemic Immune Activation and CCR5<sup>+</sup>CD4<sup>+</sup> T Cells in Filarial Driven Lymphedema in Ghana and Tanzania |
| title_full_unstemmed | Stage-Dependent Increase of Systemic Immune Activation and CCR5<sup>+</sup>CD4<sup>+</sup> T Cells in Filarial Driven Lymphedema in Ghana and Tanzania |
| title_short | Stage-Dependent Increase of Systemic Immune Activation and CCR5<sup>+</sup>CD4<sup>+</sup> T Cells in Filarial Driven Lymphedema in Ghana and Tanzania |
| title_sort | stage dependent increase of systemic immune activation and ccr5 sup sup cd4 sup sup t cells in filarial driven lymphedema in ghana and tanzania |
| topic | CD4<sup>+</sup> T cell activation lymphatic filariasis lymphedema CCR5 PD-1 HLADR/CD38 |
| url | https://www.mdpi.com/2076-0817/12/6/809 |
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