Microbial DNA extraction of high-host content and low biomass samples: Optimized protocol for nasopharynx metagenomic studies

Microbial DNA extraction of high-host content and low biomass samples: Optimized protocol for nasopharynx metagenomic studies

IntroductionLow microbial biomass and high human DNA content in nasopharyngeal aspirate samples hinder comprehensive characterization of microbiota and resistome. We obtained samples from premature infants, a group with increased risk of developing respiratory disorders and infections, and consequen...

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Bibliographic Details
Main Authors: Polona Rajar, Achal Dhariwal, Gabriela Salvadori, Roger Junges, Heidi Aarø Åmdal, Dag Berild, Drude Fugelseth, Ola Didrik Saugstad, Ulrik Lausten-Thomsen, Gorm Greisen, Kirsti Haaland, Fernanda Cristina Petersen
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-12-01
Series:Frontiers in Microbiology
Subjects:
microbiome
whole metagenomic sequencing
host DNA depletion
low biomass
respiratory microbiome
resistome
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2022.1038120/full

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https://www.frontiersin.org/articles/10.3389/fmicb.2022.1038120/full

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