Chrysosplenol D protects mice against LPS-induced acute lung injury by inhibiting oxidative stress, inflammation, and apoptosis via TLR4-MAPKs/NF-κB signaling pathways
This study investigated the effect and mechanism of chrysosplenol D (CD) on LPS-induced acute lung injury in mice. Histological changes in the lungs were measured by hematoxylin-eosin staining. The levels of IL-6, IL-1β, and TNF-α in the bronchoalveolar lavage fluid were detected by ELISA. The level...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2021-10-01
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| Series: | Innate Immunity |
| Online Access: | https://doi.org/10.1177/17534259211051069 |
| _version_ | 1797936142730919936 |
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| author | Qinqin Zhang Aozi Feng Mengnan Zeng Beibei Zhang Jingya Shi Yaxin Lv Bing Cao Chenxin Zhao Mengya Wang Yifan Ding Xiaoke Zheng |
| author_facet | Qinqin Zhang Aozi Feng Mengnan Zeng Beibei Zhang Jingya Shi Yaxin Lv Bing Cao Chenxin Zhao Mengya Wang Yifan Ding Xiaoke Zheng |
| author_sort | Qinqin Zhang |
| collection | DOAJ |
| description | This study investigated the effect and mechanism of chrysosplenol D (CD) on LPS-induced acute lung injury in mice. Histological changes in the lungs were measured by hematoxylin-eosin staining. The levels of IL-6, IL-1β, and TNF-α in the bronchoalveolar lavage fluid were detected by ELISA. The levels of oxidative stress were detected by the cuvette assay. Immune cells in peripheral blood, the levels of reactive oxygen species, and apoptosis of primary lung cells were detected by flow cytometry. The mRNA levels of TLR4, MyD88, IL-1β, and NLRP3 were measured by quantitative real-time polymerase chain reaction. The levels of proteins in apoptosis and the TLR4-MAPKs/NF-κB signaling pathways were detected by Western blot. Hematoxylin-eosin staining showed that CD could improve lung injury; decrease the levels of inflammatory factors, oxidative stress, reactive oxygen species, and cell apoptosis; and regulate the immune system. Moreover, CD could down-regulate the mRNA levels of TLR4, MyD88, NLRP3, and IL-1β in lung, and the protein levels of Keap-1, Cleaved-Caspase-3/Caspase-3, Cleaved-Caspase-9/Caspase-9, TLR4, MyD88, p-ERK/ERK, p-JNK/JNK, p-p38/p38, p-p65/p65, NLRP3, and IL-1β, and up-regulated the levels of Bcl-2/Bax, p-Nrf2/Nrf2, and HO-1. The results suggested that CD could protect mice against LPS-induced acute lung injury by inhibiting oxidative stress, inflammation, and apoptosis via the TLR4-MAPKs/NF-κB signaling pathways. |
| first_indexed | 2024-04-10T18:26:11Z |
| format | Article |
| id | doaj.art-fa583f276b694c289bb30b0099211e30 |
| institution | Directory Open Access Journal |
| issn | 1753-4259 1753-4267 |
| language | English |
| last_indexed | 2024-04-10T18:26:11Z |
| publishDate | 2021-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Innate Immunity |
| spelling | doaj.art-fa583f276b694c289bb30b0099211e302023-02-02T06:03:30ZengSAGE PublishingInnate Immunity1753-42591753-42672021-10-012710.1177/17534259211051069Chrysosplenol D protects mice against LPS-induced acute lung injury by inhibiting oxidative stress, inflammation, and apoptosis via TLR4-MAPKs/NF-κB signaling pathwaysQinqin Zhang0Aozi Feng1Mengnan Zeng2Beibei Zhang3Jingya Shi4Yaxin Lv5Bing Cao6Chenxin Zhao7Mengya Wang8Yifan Ding9Xiaoke Zheng10 The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou, China Department of Clinical Research, the First Affiliated Hospital of Jinan University, Guangzhou, China The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou, China The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou, China The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou, China The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou, China The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou, China , Zhengzhou, China , Zhengzhou, China , Zhengzhou, China The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou, ChinaThis study investigated the effect and mechanism of chrysosplenol D (CD) on LPS-induced acute lung injury in mice. Histological changes in the lungs were measured by hematoxylin-eosin staining. The levels of IL-6, IL-1β, and TNF-α in the bronchoalveolar lavage fluid were detected by ELISA. The levels of oxidative stress were detected by the cuvette assay. Immune cells in peripheral blood, the levels of reactive oxygen species, and apoptosis of primary lung cells were detected by flow cytometry. The mRNA levels of TLR4, MyD88, IL-1β, and NLRP3 were measured by quantitative real-time polymerase chain reaction. The levels of proteins in apoptosis and the TLR4-MAPKs/NF-κB signaling pathways were detected by Western blot. Hematoxylin-eosin staining showed that CD could improve lung injury; decrease the levels of inflammatory factors, oxidative stress, reactive oxygen species, and cell apoptosis; and regulate the immune system. Moreover, CD could down-regulate the mRNA levels of TLR4, MyD88, NLRP3, and IL-1β in lung, and the protein levels of Keap-1, Cleaved-Caspase-3/Caspase-3, Cleaved-Caspase-9/Caspase-9, TLR4, MyD88, p-ERK/ERK, p-JNK/JNK, p-p38/p38, p-p65/p65, NLRP3, and IL-1β, and up-regulated the levels of Bcl-2/Bax, p-Nrf2/Nrf2, and HO-1. The results suggested that CD could protect mice against LPS-induced acute lung injury by inhibiting oxidative stress, inflammation, and apoptosis via the TLR4-MAPKs/NF-κB signaling pathways.https://doi.org/10.1177/17534259211051069 |
| spellingShingle | Qinqin Zhang Aozi Feng Mengnan Zeng Beibei Zhang Jingya Shi Yaxin Lv Bing Cao Chenxin Zhao Mengya Wang Yifan Ding Xiaoke Zheng Chrysosplenol D protects mice against LPS-induced acute lung injury by inhibiting oxidative stress, inflammation, and apoptosis via TLR4-MAPKs/NF-κB signaling pathways Innate Immunity |
| title | Chrysosplenol D protects mice against LPS-induced acute lung injury by inhibiting oxidative stress, inflammation, and apoptosis via TLR4-MAPKs/NF-κB signaling pathways |
| title_full | Chrysosplenol D protects mice against LPS-induced acute lung injury by inhibiting oxidative stress, inflammation, and apoptosis via TLR4-MAPKs/NF-κB signaling pathways |
| title_fullStr | Chrysosplenol D protects mice against LPS-induced acute lung injury by inhibiting oxidative stress, inflammation, and apoptosis via TLR4-MAPKs/NF-κB signaling pathways |
| title_full_unstemmed | Chrysosplenol D protects mice against LPS-induced acute lung injury by inhibiting oxidative stress, inflammation, and apoptosis via TLR4-MAPKs/NF-κB signaling pathways |
| title_short | Chrysosplenol D protects mice against LPS-induced acute lung injury by inhibiting oxidative stress, inflammation, and apoptosis via TLR4-MAPKs/NF-κB signaling pathways |
| title_sort | chrysosplenol d protects mice against lps induced acute lung injury by inhibiting oxidative stress inflammation and apoptosis via tlr4 mapks nf κb signaling pathways |
| url | https://doi.org/10.1177/17534259211051069 |
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