Quantitative and qualitative analysis of three DNA extraction methods from soybean, maize, and canola oils and investigation of the presence of genetically modified organisms (GMOs)
The objective of this study was to develop a DNA-based method for the identification and tracking of edible oils, which is important for health management. Three different DNA extraction methods (CTAB, MBST kit, and manual hexane-based method) were used to obtain high-purity DNA from crude and refin...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-07-01
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| Series: | Food Chemistry: Molecular Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266656622400008X |
| _version_ | 1827223395613278208 |
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| author | Melika Vahdani Mohammad Ali Sahari Mehrnaz Tanavar |
| author_facet | Melika Vahdani Mohammad Ali Sahari Mehrnaz Tanavar |
| author_sort | Melika Vahdani |
| collection | DOAJ |
| description | The objective of this study was to develop a DNA-based method for the identification and tracking of edible oils, which is important for health management. Three different DNA extraction methods (CTAB, MBST kit, and manual hexane-based method) were used to obtain high-purity DNA from crude and refined soybean, maize, and canola oils. PCR was then conducted using specific primers to identify the presence of genes related to each oil type and to assess transgenicity. The results showed that DNA was present in crude and refined oils, but in very low amounts. However, using method 3 for DNA extraction provided sufficient quantity and quality of DNA for successful PCR amplification. The study concluded that the main challenge in DNA extraction from oils is the presence of PCR inhibitors, which can be overcome using the manual hexane-based method. Also, the examination of protein presence in the oils using SDS-PAGE did not indicate any protein bands. |
| first_indexed | 2024-04-24T16:07:10Z |
| format | Article |
| id | doaj.art-fa59c3b2254c4a6db3b4056c0ae898ef |
| institution | Directory Open Access Journal |
| issn | 2666-5662 |
| language | English |
| last_indexed | 2025-03-21T16:53:42Z |
| publishDate | 2024-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: Molecular Sciences |
| spelling | doaj.art-fa59c3b2254c4a6db3b4056c0ae898ef2024-06-15T06:13:50ZengElsevierFood Chemistry: Molecular Sciences2666-56622024-07-018100201Quantitative and qualitative analysis of three DNA extraction methods from soybean, maize, and canola oils and investigation of the presence of genetically modified organisms (GMOs)Melika Vahdani0Mohammad Ali Sahari1Mehrnaz Tanavar2Department of Food Science and Technology, College of Agriculture, Tarbiat Modares University, P. O. Box, 14115-336 Tehran, IranDepartment of Food Science and Technology, College of Agriculture, Tarbiat Modares University, P. O. Box, 14115-336 Tehran, Iran; Corresponding author.Department of Plant Genetics and Breeding, College of Agriculture, Tarbiat Modares University, Tehran, IranThe objective of this study was to develop a DNA-based method for the identification and tracking of edible oils, which is important for health management. Three different DNA extraction methods (CTAB, MBST kit, and manual hexane-based method) were used to obtain high-purity DNA from crude and refined soybean, maize, and canola oils. PCR was then conducted using specific primers to identify the presence of genes related to each oil type and to assess transgenicity. The results showed that DNA was present in crude and refined oils, but in very low amounts. However, using method 3 for DNA extraction provided sufficient quantity and quality of DNA for successful PCR amplification. The study concluded that the main challenge in DNA extraction from oils is the presence of PCR inhibitors, which can be overcome using the manual hexane-based method. Also, the examination of protein presence in the oils using SDS-PAGE did not indicate any protein bands.http://www.sciencedirect.com/science/article/pii/S266656622400008XGenetically modified organisms (GMOs)Polymerase chain reaction (PCR)DNA extractionPCR inhibitorsCrude and refined edible oils |
| spellingShingle | Melika Vahdani Mohammad Ali Sahari Mehrnaz Tanavar Quantitative and qualitative analysis of three DNA extraction methods from soybean, maize, and canola oils and investigation of the presence of genetically modified organisms (GMOs) Food Chemistry: Molecular Sciences Genetically modified organisms (GMOs) Polymerase chain reaction (PCR) DNA extraction PCR inhibitors Crude and refined edible oils |
| title | Quantitative and qualitative analysis of three DNA extraction methods from soybean, maize, and canola oils and investigation of the presence of genetically modified organisms (GMOs) |
| title_full | Quantitative and qualitative analysis of three DNA extraction methods from soybean, maize, and canola oils and investigation of the presence of genetically modified organisms (GMOs) |
| title_fullStr | Quantitative and qualitative analysis of three DNA extraction methods from soybean, maize, and canola oils and investigation of the presence of genetically modified organisms (GMOs) |
| title_full_unstemmed | Quantitative and qualitative analysis of three DNA extraction methods from soybean, maize, and canola oils and investigation of the presence of genetically modified organisms (GMOs) |
| title_short | Quantitative and qualitative analysis of three DNA extraction methods from soybean, maize, and canola oils and investigation of the presence of genetically modified organisms (GMOs) |
| title_sort | quantitative and qualitative analysis of three dna extraction methods from soybean maize and canola oils and investigation of the presence of genetically modified organisms gmos |
| topic | Genetically modified organisms (GMOs) Polymerase chain reaction (PCR) DNA extraction PCR inhibitors Crude and refined edible oils |
| url | http://www.sciencedirect.com/science/article/pii/S266656622400008X |
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