Application of plackett-burman experimental design to optimize the cold-active alpha amylase biosynthesis by psychrotrophic Streptomyces 4 Alga
The aim of this work was to optimize the cultural and production parameters through the statistical approach for the synthesis of cold active alpha amylase by Streptomyces 4 Alga in submerged fermentation. The process parameters influencing the enzyme production were identified using Plackett-Burman...
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| Format: | Article |
| Language: | English |
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Galati University Press
2012-12-01
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| Series: | Annals of the University Dunarea de Jos of Galati. Fascicle VI: Food Technology |
| Subjects: | |
| Online Access: | http://www.ann.ugal.ro/tpa/Anale%202012/vol2/1_MCotarlet.pdf |
| _version_ | 1818847964721840128 |
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| author | Mihaela COTÂRLEŢ Gabriela Elena BAHRIM |
| author_facet | Mihaela COTÂRLEŢ Gabriela Elena BAHRIM |
| author_sort | Mihaela COTÂRLEŢ |
| collection | DOAJ |
| description | The aim of this work was to optimize the cultural and production parameters through the statistical approach for the synthesis of cold active alpha amylase by Streptomyces 4 Alga in submerged fermentation. The process parameters influencing the enzyme production were identified using Plackett-Burman design. Among the various variables screened, the yeast extract and CaCl2 were the most significant. The optimum levels of these significant parameterswere determined employing the Response Surface Methodology and theCentral Composite Design. The most significant variables were determined as follows: yeast extract (5.00 g%) and CaCl2 (0.25 g%). By using the optimal fermentation medium, the cold-active alpha amylase production was increased up to 23.96 AU, an approximate 4.4-fold improvement over the previous production (5.44 AU) with un-optimized medium. The cold-active alpha amylases could be used in bioethanol production at lower temperatures, waste-water treatment and bioremediation in cold climates. |
| first_indexed | 2024-12-19T06:09:49Z |
| format | Article |
| id | doaj.art-fa6a495cbdf14c96844346fdd037a414 |
| institution | Directory Open Access Journal |
| issn | 1843-5157 2068-259X |
| language | English |
| last_indexed | 2024-12-19T06:09:49Z |
| publishDate | 2012-12-01 |
| publisher | Galati University Press |
| record_format | Article |
| series | Annals of the University Dunarea de Jos of Galati. Fascicle VI: Food Technology |
| spelling | doaj.art-fa6a495cbdf14c96844346fdd037a4142022-12-21T20:33:03ZengGalati University PressAnnals of the University Dunarea de Jos of Galati. Fascicle VI: Food Technology1843-51572068-259X2012-12-01362919Application of plackett-burman experimental design to optimize the cold-active alpha amylase biosynthesis by psychrotrophic Streptomyces 4 AlgaMihaela COTÂRLEŢGabriela Elena BAHRIMThe aim of this work was to optimize the cultural and production parameters through the statistical approach for the synthesis of cold active alpha amylase by Streptomyces 4 Alga in submerged fermentation. The process parameters influencing the enzyme production were identified using Plackett-Burman design. Among the various variables screened, the yeast extract and CaCl2 were the most significant. The optimum levels of these significant parameterswere determined employing the Response Surface Methodology and theCentral Composite Design. The most significant variables were determined as follows: yeast extract (5.00 g%) and CaCl2 (0.25 g%). By using the optimal fermentation medium, the cold-active alpha amylase production was increased up to 23.96 AU, an approximate 4.4-fold improvement over the previous production (5.44 AU) with un-optimized medium. The cold-active alpha amylases could be used in bioethanol production at lower temperatures, waste-water treatment and bioremediation in cold climates.http://www.ann.ugal.ro/tpa/Anale%202012/vol2/1_MCotarlet.pdfStreptomyces sp.alpha amylaseresponse surface methodology (RSM)submerged fermentationPlackett-Burman designcold-adapted alpha amylase production |
| spellingShingle | Mihaela COTÂRLEŢ Gabriela Elena BAHRIM Application of plackett-burman experimental design to optimize the cold-active alpha amylase biosynthesis by psychrotrophic Streptomyces 4 Alga Annals of the University Dunarea de Jos of Galati. Fascicle VI: Food Technology Streptomyces sp. alpha amylase response surface methodology (RSM) submerged fermentation Plackett-Burman design cold-adapted alpha amylase production |
| title | Application of plackett-burman experimental design to optimize the cold-active alpha amylase biosynthesis by psychrotrophic Streptomyces 4 Alga |
| title_full | Application of plackett-burman experimental design to optimize the cold-active alpha amylase biosynthesis by psychrotrophic Streptomyces 4 Alga |
| title_fullStr | Application of plackett-burman experimental design to optimize the cold-active alpha amylase biosynthesis by psychrotrophic Streptomyces 4 Alga |
| title_full_unstemmed | Application of plackett-burman experimental design to optimize the cold-active alpha amylase biosynthesis by psychrotrophic Streptomyces 4 Alga |
| title_short | Application of plackett-burman experimental design to optimize the cold-active alpha amylase biosynthesis by psychrotrophic Streptomyces 4 Alga |
| title_sort | application of plackett burman experimental design to optimize the cold active alpha amylase biosynthesis by psychrotrophic streptomyces 4 alga |
| topic | Streptomyces sp. alpha amylase response surface methodology (RSM) submerged fermentation Plackett-Burman design cold-adapted alpha amylase production |
| url | http://www.ann.ugal.ro/tpa/Anale%202012/vol2/1_MCotarlet.pdf |
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