Recombinant expression, purification and characterization of Bombyx mori (Lepidoptera: Bombycidae) pyridoxal kinase

Recombinant expression, purification and characterization of Bombyx mori (Lepidoptera: Bombycidae) pyridoxal kinase

Pyridoxal kinase (PLK; EC 2.7.1.35) is a key enzyme in the metabolism of vitamin B6 (VB6) in Bombyx mori. A fusion expressional vector pET-22b-BPLK-His was constructed using a sub-cloning technique, the recombinant B. mori PLK was then expressed in Escherichia coli, purified and characterized. Bioin...

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Bibliographic Details
Main Authors: Shuo-Hao HUANG, Wang MA, Ping-Ping ZHANG, Jian-Yun ZHANG, Yan-Feng XIE, Long-Quan HUANG
Format: Article
Language:English
Published: Institute of Entomology, Biology Centre, Czech Academy of Science 2011-01-01
Series:European Journal of Entomology
Subjects:
bombyx mori
pyridoxal kinase
recombinant expression
purification
characterization
Online Access:https://www.eje.cz/artkey/eje-201101-0003_Recombinant_expression_purification_and_characterization_of_Bombyx_mori_Lepidoptera_Bombycidae_pyridoxal_ki.php

Internet

https://www.eje.cz/artkey/eje-201101-0003_Recombinant_expression_purification_and_characterization_of_Bombyx_mori_Lepidoptera_Bombycidae_pyridoxal_ki.php

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