Nucleolus localization of SpyCas9 affects its stability and interferes with host protein translation in mammalian cells

Nucleolus localization of SpyCas9 affects its stability and interferes with host protein translation in mammalian cells

The CRISPR/Cas9 system, originally derived from the prokaryotic adaptive immune system, has been developed as efficient genome editing tools. It enables precise gene manipulation on chromosomal DNA through the specific binding of programmable sgRNA to target DNA, and the Cas9 protein, which has endo...

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Bibliographic Details
Main Authors: Renke Tan, Wenhao Du, Yiyang Liu, Xiaoji Cong, Meirong Bai, Chenxiao Jiang, Zengxia Li, Minjia Tan, Dengke K. Ma, Qiang Huang, Wei Jiang, Yongjun Dang
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2022-05-01
Series:Genes and Diseases
Subjects:
CRISPR/Cas9
Gene editing
Global transcription
Nucleolus detention signal
Translation
Online Access:http://www.sciencedirect.com/science/article/pii/S2352304220301215

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http://www.sciencedirect.com/science/article/pii/S2352304220301215

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